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Investigations of multivalent ligand-receptor interactions in microfluidic devices
dc.creator | Baryshnikova, Olga K. | |
dc.date.accessioned | 2012-06-07T23:11:38Z | |
dc.date.available | 2012-06-07T23:11:38Z | |
dc.date.created | 2002 | |
dc.date.issued | 2002 | |
dc.identifier.uri | https://hdl.handle.net/1969.1/ETD-TAMU-2002-THESIS-B386 | |
dc.description | Due to the character of the original source materials and the nature of batch digitization, quality control issues may be present in this document. Please report any quality issues you encounter to digital@library.tamu.edu, referencing the URI of the item. | en |
dc.description | Includes bibliographical references (leaves 42-45). | en |
dc.description | Issued also on microfiche from Lange Micrographics. | en |
dc.description.abstract | The objective of the studies presented herein was to obtain thermodynamic data on surface confined multivalent ligand - receptor interactions. Novel microfluidic strategies in conjunction with total internal reflection fluorescence microscopy (TIRFM) were designed in order to achieve this. Concanavalin A bound to α-manno-sylated lipids on supported phospholipid bilayers was chosen as a model bivalent system. The acquired binding parameters on bilayers containing various ligand densities were examined by modifying available thermodynamic models. In the case of bivalent systems, two individual dissociation constants could be extracted from the experimental data. The values of these parameters demonstrated significant binding enhancement due to the presence of a fluid membrane interface. | en |
dc.format.medium | electronic | en |
dc.format.mimetype | application/pdf | |
dc.language.iso | en_US | |
dc.publisher | Texas A&M University | |
dc.rights | This thesis was part of a retrospective digitization project authorized by the Texas A&M University Libraries in 2008. Copyright remains vested with the author(s). It is the user's responsibility to secure permission from the copyright holder(s) for re-use of the work beyond the provision of Fair Use. | en |
dc.subject | chemistry. | en |
dc.subject | Major chemistry. | en |
dc.title | Investigations of multivalent ligand-receptor interactions in microfluidic devices | en |
dc.type | Thesis | en |
thesis.degree.discipline | chemistry | en |
thesis.degree.name | M.S. | en |
thesis.degree.level | Masters | en |
dc.type.genre | thesis | en |
dc.type.material | text | en |
dc.format.digitalOrigin | reformatted digital | en |
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