Abstract
The objective of the studies presented herein was to obtain thermodynamic data on surface confined multivalent ligand - receptor interactions. Novel microfluidic strategies in conjunction with total internal reflection fluorescence microscopy (TIRFM) were designed in order to achieve this. Concanavalin A bound to α-manno-sylated lipids on supported phospholipid bilayers was chosen as a model bivalent system. The acquired binding parameters on bilayers containing various ligand densities were examined by modifying available thermodynamic models. In the case of bivalent systems, two individual dissociation constants could be extracted from the experimental data. The values of these parameters demonstrated significant binding enhancement due to the presence of a fluid membrane interface.
Baryshnikova, Olga K. (2002). Investigations of multivalent ligand-receptor interactions in microfluidic devices. Master's thesis, Texas A&M University. Available electronically from
https : / /hdl .handle .net /1969 .1 /ETD -TAMU -2002 -THESIS -B386.