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Correlation between leukotoxin production of Pasteurella haemolytica and bacterial culture conditions
| dc.creator | Fann, Hsing Fu | |
| dc.date.accessioned | 2012-06-07T22:36:15Z | |
| dc.date.available | 2012-06-07T22:36:15Z | |
| dc.date.created | 1994 | |
| dc.date.issued | 1994 | |
| dc.identifier.uri | https://hdl.handle.net/1969.1/ETD-TAMU-1994-THESIS-F2134 | |
| dc.description | Due to the character of the original source materials and the nature of batch digitization, quality control issues may be present in this document. Please report any quality issues you encounter to digital@library.tamu.edu, referencing the URI of the item. | en |
| dc.description | Includes bibliographical references. | en |
| dc.description.abstract | A study of the in vitro growth characteristics of Pasteurella haemolytica was undertaken to determine the effect of media composition and available oxygen on bacterial growth. The rate and total growth, and the production of the exotoxin, leukotoxin, were measured over time. Two basic culture media, brain heart infusion broth and Roswell Park Memorial Institute (RPMI) medium 1640, were used. For the RPMI 1640 culture media, sodium bicarbonate (NaHCO3) was used as the buffer system. Media supplements included fetal bovine serum (FBS), fresh bovine plasma (FBP), fresh bovine blood cells, both red blood cells (RBC) and white blood cells (WBC) dextrose and yeast extract. The effect of available oxygen was studied in cultures with large air exchange surfaces as compared to cultures with restricted air exchange. Each media supplement, individually and in combination, increased both the rate and total amount of bacterial growth and the total leukotoxin production. Fresh bovine plasma was a better supplement than fetal bovine serum and both fresh living and killed bovine WBC stimulated leukotoxin production. | en |
| dc.format.medium | electronic | en |
| dc.format.mimetype | application/pdf | |
| dc.language.iso | en_US | |
| dc.publisher | Texas A&M University | |
| dc.rights | This thesis was part of a retrospective digitization project authorized by the Texas A&M University Libraries in 2008. Copyright remains vested with the author(s). It is the user's responsibility to secure permission from the copyright holder(s) for re-use of the work beyond the provision of Fair Use. | en |
| dc.subject | veterinary pathobiology. | en |
| dc.subject | Major veterinary pathobiology. | en |
| dc.title | Correlation between leukotoxin production of Pasteurella haemolytica and bacterial culture conditions | en |
| dc.type | Thesis | en |
| thesis.degree.discipline | veterinary pathobiology | en |
| thesis.degree.name | M.S. | en |
| thesis.degree.level | Masters | en |
| dc.type.genre | thesis | en |
| dc.type.material | text | en |
| dc.format.digitalOrigin | reformatted digital | en |
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