Abstract
A study of the in vitro growth characteristics of Pasteurella haemolytica was undertaken to determine the effect of media composition and available oxygen on bacterial growth. The rate and total growth, and the production of the exotoxin, leukotoxin, were measured over time. Two basic culture media, brain heart infusion broth and Roswell Park Memorial Institute (RPMI) medium 1640, were used. For the RPMI 1640 culture media, sodium bicarbonate (NaHCO3) was used as the buffer system. Media supplements included fetal bovine serum (FBS), fresh bovine plasma (FBP), fresh bovine blood cells, both red blood cells (RBC) and white blood cells (WBC) dextrose and yeast extract. The effect of available oxygen was studied in cultures with large air exchange surfaces as compared to cultures with restricted air exchange. Each media supplement, individually and in combination, increased both the rate and total amount of bacterial growth and the total leukotoxin production. Fresh bovine plasma was a better supplement than fetal bovine serum and both fresh living and killed bovine WBC stimulated leukotoxin production.
Fann, Hsing Fu (1994). Correlation between leukotoxin production of Pasteurella haemolytica and bacterial culture conditions. Master's thesis, Texas A&M University. Available electronically from
https : / /hdl .handle .net /1969 .1 /ETD -TAMU -1994 -THESIS -F2134.