Abstract
The left sides of six mature (USDA E-Maturity) cattle were electrically stimulated with 18 impulses of approximately 500 volts. The right sides of the carcasses served as controls (NS). Samples (200 g) were excised at 0 and 6 hr postmortem and were subjected to sarcoplasmic reticulum isolation procedure. Isolated sarcoplasmic reticulum (SR) vesicles were subsequently used for calcium uptake and efflux determinations using ('45)Ca as the label. Results indicated that Ca('++) accumulation by SR vesicles isolated from electrically stimulated muscle as 6 hr postmortem, was reduced by approximately 45-55% when compared to the other samples, ES-0, NS-0, and NS-6. The yield of SR protein from muscle, however, was not greatly affected by either electrical stimulation or postmortem storage time. Calcium release from isolated SR vesicles which had been previously loaded with Ca('++), did not appear to be substantially affected by the addition of 2.5 mM ADP to the reaction buffer. However, a rapid and irreversible loss of Ca('++) from previously loaded SR vesicles was produced following the addition of 100 mM ADP to the reaction buffer. ...
Joseph, Amy Louise (1980). The effect of electrical stimulation and postmortem time on properties of the sarcoplasmic reticulum. Texas A&M University. Texas A&M University. Libraries. Available electronically from
https : / /hdl .handle .net /1969 .1 /DISSERTATIONS -685780.