The Effects of Varying Glucose Media Concentration on Spores of Bacillus thuringiensis var. kurstaki HD-1
Abstract
Bacillus thuringiensis spores were grown on nonsupplemented, 0.1, 0.5, or 1.0 percent glucose supplemented nutrient agar. Spores were isolated using a density gradient of Angiovist. The spore coats were removed through solubilization with a Tris buffer containing sodium dodecyl sulfate. Soluble versus total protein content for spores from each concentration was then analyzed by a dye-binding technique and the respective ratios were calculated. Sodium dodecyl sulfate polyacrylamide gel electrophoresis further analyzed the soluble and the total spore proteins.
Description
Program year: 1996/1997Digitized from print original stored in HDR
Citation
Litzinger, Jill (1987). The Effects of Varying Glucose Media Concentration on Spores of Bacillus thuringiensis var. kurstaki HD-1. University Undergraduate Fellow. Available electronically from https : / /hdl .handle .net /1969 .1 /CAPSTONE -LitzingerJ _1987.