Bioinformatic method for identifying surface-anchored proteins from gram-positive bacteria and proteins obtained thereby
Abstract
A bioinformatic method is provided for identifying and isolating proteins with MSCRAMM®—like characteristics from Gram positive bacteria, such as Enterococcus, Staphylococcus, Streptococcus and Bacillus bacteria, which can then be utilized in methods to prevent and treat infections caused by Gram-positive bacteria. The method involves identifying from sequence information those proteins with a putative C-terminal LPXTG (SEQ ID NO:1) cell wall sorting signal and other structural similarities to MSCRAMM® proteins having the LPXTG-anchored cell wall proteins. The MSCRAMM® proteins and immunogenic regions therein that are identified and isolated using the present invention may be used to generate antibodies useful in the diagnosis, treatment or prevention of Gram positive bacterial infections.
Subject
530/387.1424/130.1
424/133.1
424/139.1
424/141.1
424/150.1
424/164.1
435/243
435/975
530/388.1
G01N 33/56911
C07K 14/315
C07K 16/1267
A61P 37/04
G01N 33/56916
A61P 31/04
G16B 30/00
A61K 38/00
Y02A 90/10
Y02A 90/26
G16B 15/00
Y10S 435/975
Collections
Citation
Hook, Magnus; Xu, Yi; Sillanpaa, Jouko V.; Sthanam, Narayana; Ponnuraj, Karthe; Patti, Joseph M.; Hutchins, Jeff T.; Hall, Andrea; Bowden, Maria G. (2009). Bioinformatic method for identifying surface-anchored proteins from gram-positive bacteria and proteins obtained thereby. United States. Patent and Trademark Office; Texas A&M University. Libraries. Available electronically from https : / /hdl .handle .net /1969 .1 /176904.