Using CRISPR to Knockout RPA3B in Arabidopsis Thaliana
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Replication Protein A (RPA) is a heterotrimeric single stranded DNA binding (SSB) protein. RPA has functions in DNA metabolism and meiosis (1). Specifically, the subunit RPA3 helps give structure to the heterotrimeric complex, allowing the RPA1 subunit to bind to DNA in order to perform various reactions involved in DNA replication and repair. To further characterize the activity of RPA3B, knockouts will be generated in Arabidopsis thaliana using CRISPR, a gene editing technology that has become widely used recently.
Fatora, John Stephen (2019). Using CRISPR to Knockout RPA3B in Arabidopsis Thaliana. Undergraduate Research Scholars Program. Available electronically from