Browsing by Subject "CRISPR"
Now showing items 1-12 of 12
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(2022-12-12)During C. elegans development, EGF signal from the anchor cell (AC) induces the roughly six equipotent vulval precursor cells (VPCs) to assume the pattern 3˚-3˚-2˚-1˚-2˚-3˚ of cell fates. The VPC closest to the AC is induced ...
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(2022-09-13)Rice is an essential food crop providing the daily caloric intake for over 50% of the world’s population. Flowering is one of the most sensitive stages of rice growth and is highly variable among varieties and across ...
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(2019-07-11)The CRISPR/Cas9 gene editing platform is capable of inducing double-stranded breaks in a targeted genomic region using a sequence specific single guide RNA (sgRNA) and programmable Cas9 endonuclease. Plant viral vectors ...
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(2021-03-22)Aedes aegypti is a vector of dengue, chikungunya, and Zika viruses. Current vector control strategies such as community engagement, source reduction, and insecticides have not been sufficient to prevent viral outbreaks. ...
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(2017-04-28)Disease decreases production efficiency and kills millions of animals across all types of livestock operations every year. This is severely detrimental to both animal producers and consumers from an economic and overall ...
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(2019-08-14)The newly-discovered Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system has enabled rapid genome editing in plants. Moreover, CRISPR/Cas9-based editing opens up the ...
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(2020-09-11)One of the main hubs of cellular metabolic integration is the mechanistic target of rapamycin, or mTOR. In normal, healthy cells, mTOR helps to regulate and balance cellular growth and proliferation against metabolic ...
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PRECISE AND EFFICIENT THERAPEUTIC GENOME EDITING FOR THE CORRECTION OF GENETIC DISEASES IN ANIMALS (2017-12-12)There are more than 6,052 identified genetic mutations linked to disease in humans and animals. Thanks to the advent of gene editing based on programmable nucleases and the advances in DNA sequencing and writing technologies, ...
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(2021-05-19)CRISPR-Cas9 mutagenesis has been developed and proved to function in the bacterium Clostridioides difficile, albeit with efficiency and ease-of-use below the expected level. Here, we pursue two separate methods of improving ...
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In this paper, I will be assisting in developing a new method of characterizing communication between the enhancers and promoter within the fgf8a gene in vertebrates, specifically in zebrafish. The fgf8a gene controls ...
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(2022-11-07)Aedes aegypti is a mosquito species of high medical relevance as a vector of arboviruses such as dengue, yellow fever, Zika, and chikungunya. Vector control via drainage and insecticide use has been the main strategy to ...
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Replication Protein A (RPA) is a heterotrimeric single stranded DNA binding (SSB) protein. RPA has functions in DNA metabolism and meiosis (1). Specifically, the subunit RPA3 helps give structure to the heterotrimeric ...