Engineered Split-TET2 Enzyme for Inducible Epigenetic Remodeling
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The conversion of 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and further oxidized species is catalyzed by the Ten-eleven translocation (TET) methylcytosine dioxygenase. Here we provided a temporal control of 5mC oxidation and subsequent remodeling of epigenetic states through a split-TET2 enzyme system in mammalian cells. This chemical-inducible epigenome remodeling tool provides a precise way to interrogate cellular systems and probe the epigenotype-phenotype relations in various biological systems.
Liang, Yi (2017). Engineered Split-TET2 Enzyme for Inducible Epigenetic Remodeling. Master's thesis, Texas A & M University. Available electronically from