Purifying an Unknown Burkholderia contaminans MS14 Bactericidal
Recently, the rapid pace at which bacteria can successfully evade antibiotics has alarmed public health officials, since they could render even our most powerful antibiotics useless. With this in mind, it is evident to see the value and urgency in discovering novel antimicrobials. This project’s focus is on the isolation and purification of an unknown bactericidal compound from B. contaminans MS14. While attempting to isolate and purify the unknown bactericidal agent, an interesting discovery was found. Ornibactin, a siderophore produced by MS14, has been shown to play a significant role in the bactericidal activity of the unknown antimicrobial. These two MS14 products seem to be working in tandem in some way, which reveals an interesting mode of action for our target antimicrobial.
Herrera, Keren (2017). Purifying an Unknown Burkholderia contaminans MS14 Bactericidal. Undergraduate Research Scholars Program. Available electronically from