Data from: Cytochrome P450 Inhibitors Reduce Creeping Bentgrass (Agrostis stolonifera) Tolerance to Topramezone

Abstract

Creeping bentgrass (Agrostis stolonifera L.) is moderately tolerant to the p-hydroxyphenylpyruvate dioxygenase-inhibiting herbicide topramezone. However, the contribution of plant metabolism of topramezone metabolism to this tolerance is unknown. Experiments were conducted to determine if known cytochrome P450 monooxygenase inhibitors 1-aminobenzotriazole (ABT) and malathion alone or in combination with the herbicide safener cloquintocet-mexyl influence creeping bentgrass tolerance to topramezone. Creeping bentgrass in hydroponic culture was treated with ABT (70 μm), malathion (70 μm and 1000 g ha-1), or cloquintocet-mexyl (70 μm and 1000 g ha-1) prior to topramezone (8 g ha-1) application. Topramezone-induced injury to creeping bentgrass increased from 22% when applied alone to 79 and 41% when applied with malathion or ABT, respectively. Cloquintocet (70 μm and 1000 g ha-1) reduced topramezone injury to 1%. Cloquintocet mitigated the synergistic effects of ABT more than those of malathion. Visible responses were supported by PSII quantum yield and creeping bentgrass biomass responses. Responses to ABT and malathion suggest that creeping bentgrass tolerance to topramezone is influenced by cytochrome P450-catalyzed metabolism. Future research should determine the relative contributions of cytochrome P450 monooxygenases and glutathione-s-transferases to topramezone metabolism in creeping bentgrass.