dc.creator | Cohen, Noah D. | |
dc.creator | Bourquin, Jessica R | |
dc.creator | Bordin, Angela I. | |
dc.creator | Kuskie, Kyle R. | |
dc.creator | Brake, Courtney N. | |
dc.creator | Weaver, Kaytee B. | |
dc.creator | Liu, Mei | |
dc.creator | Felippe, M. Julia B. | |
dc.creator | Kogut, Michael H. | |
dc.date.accessioned | 2014-11-18T21:06:01Z | |
dc.date.available | 2014-11-18T21:06:01Z | |
dc.date.issued | 2014-10-15 | |
dc.identifier.citation | Cohen ND, Bourquin JR, Bordin AI, Kuskie KR, Brake CN, et al. (2014) Intramuscular Administration of a Synthetic CpG-Oligodeoxynucleotide Modulates Functional Responses of Neutrophils of Neonatal Foals. PLoS ONE 9(10): e109865. doi:10.1371/journal.pone.0109865 | en |
dc.identifier.uri | http://dx.doi.org/10.1371/journal.pone.0109865 | |
dc.identifier.uri | https://hdl.handle.net/1969.1/152271 | |
dc.description.abstract | Neutrophils play an important role in protecting against infection. Foals have age-dependent deficiencies in neutrophil function that may contribute to their predisposition to infection. Thus, we investigated the ability of a CpG-ODN formulated with Emulsigen to modulate functional responses of neutrophils in neonatal foals. Eighteen foals were randomly assigned to receive either a CpG-ODN with Emulsigen (N = 9) or saline intramuscularly at ages 1 and 7 days. At ages 1, 3, 9, 14, and 28, blood was collected and neutrophils were isolated from each foal. Neutrophils were assessed for basal and Rhodococcus equi-stimulated mRNA expression of the cytokines interferon-γ (IFN-γ), interleukin (IL)-4, IL-6, and IL-8 using real-time PCR, degranulation by quantifying the amount of β-D glucuronidase activity, and reactive oxygen species (ROS) generation using flow cytometry. In vivo administration of the CpG-ODN formulation on days 1 and 7 resulted in significantly (P<0.05) increased IFN-γ mRNA expression by foal neutrophils on days 3, 9, and 14. Degranulation was significantly (P<0.05) lower for foals in the CpG-ODN-treated group than the control group at days 3 and 14, but not at other days. No effect of treatment on ROS generation was detected. These results indicate that CpG-ODN administration to foals might improve innate and adaptive immune responses that could protect foals against infectious diseases and possibly improve responses to vaccination. | en |
dc.description.sponsorship | The open access fee for this work was funded through the Texas A&M University Open Access to Knowledge (OAK) Fund. | en |
dc.language.iso | en_US | |
dc.publisher | PLOS ONE | |
dc.rights | CC0 1.0 Universal | en |
dc.rights.uri | http://creativecommons.org/publicdomain/zero/1.0/ | |
dc.title | Intramuscular Administration of a Synthetic CpG-Oligodeoxynucleotide Modulates Functional Responses of Neutrophils of Neonatal Foals | en |
dc.type | Article | en |
local.department | Large Animal Clinical Sciences | en |
dc.rights.requestable | false | en |