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Construction and characterization of a peach binary bacterial artificial chromosome library
|dc.description||Due to the character of the original source materials and the nature of batch digitization, quality control issues may be present in this document. Please report any quality issues you encounter to firstname.lastname@example.org, referencing the URI of the item.||en_US|
|dc.description||Includes bibliographical references (leaves 35-43).||en_US|
|dc.description||Issued also on microfiche from Lange Micrographics.||en_US|
|dc.description.abstract||Peach [Prunus persica (L.) Batch] has been proposed as a model organism for woody perennials. To facilitate genomic research in peach, a binary bacterial artificial chromosome (BIBAC) library of var. Texking was constructed using a plant-transformation-competent vector, pCLD0451. Texking is a medium chill peach used in the Texas A&M University Prunus breeding program that displays valuable agronomic traits. The library was constructed in the BamH I site of the pCLD0451 vector and arrayed in 40 384-well microplates. It is comprised of 15,360 clones, having an average insert size of 123.6 kb. Of the 15,360 clones in the library, 768 (5%) were found to have no inserts. Screening with chloroplast probes from barley indicated that 32 (0.22%) of the library originated from the chloroplast genome. Therefore, 14,557 clones of the library originated from the nuclear genome, representing 6.8 X haploid genomes of the species. The BIBAC library was further screened with 8 RFLP markers selected from the peach genetic map. Library screening with the markers showed an average of 4.5 positive clones per marker, with a range of 1 to 11 positive clones. The library represents the first plant-transformation-competent BIBAC library for P. persica and will facilitate genomic research in peach and related species.||en_US|
|dc.publisher||Texas A&M University||en_US|
|dc.rights||This thesis was part of a retrospective digitization project authorized by the Texas A&M University Libraries in 2008. Copyright remains vested with the author(s). It is the user's responsibility to secure permission from the copyright holder(s) for re-use of the work beyond the provision of Fair Use.||en_US|
|dc.subject||Major plant breeding.||en_US|
|dc.title||Construction and characterization of a peach binary bacterial artificial chromosome library||en_US|
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