NOTE: This item is not available outside the Texas A&M University network. Texas A&M affiliated users who are off campus can access the item through NetID and password authentication or by using TAMU VPN. Non-affiliated individuals should request a copy through their local library's interlibrary loan service.
Attempted oral vaccination of swine with Brucella neotomae for protection against subsequent challenge with Brucella suis
dc.creator | Turner, Kenneth Edward | |
dc.date.accessioned | 2012-06-07T23:09:43Z | |
dc.date.available | 2012-06-07T23:09:43Z | |
dc.date.created | 2001 | |
dc.date.issued | 2001 | |
dc.identifier.uri | https://hdl.handle.net/1969.1/ETD-TAMU-2001-THESIS-T875 | |
dc.description | Due to the character of the original source materials and the nature of batch digitization, quality control issues may be present in this document. Please report any quality issues you encounter to digital@library.tamu.edu, referencing the URI of the item. | en |
dc.description | Includes bibliographical references (leaves 37-55). | en |
dc.description | Issued also on microfiche from Lange Micrographics. | en |
dc.description.abstract | As part of a program to develop an oral vaccine for the control of brucellosis in feral swine, 64 pregnant, domestic swine were orally exposed to Brucella neotomae and subsequently conjunctivally challenged with Brucella suis. In an effort to reduce the number of swine shedding viable Brucella sp. bacteria at any one time, two sets of swine were used in this experiment. The first set of swine consisted of 17 vaccinated and 17 non-vaccinated control animals. The second set consisted of 15 animals in each group. The vaccination procedure involved feeding the animals to be vaccinated a standard swine ration mixed with approximately 1x10¹³ colony forming units (CFU) of B. neotomae per gilt daily for 3 days, with a total average dose of >4x10¹³ CFU of B. neotomae per gilt. All animals were challenged via bi-lateral conjunctival inoculation with approximately 1x10⁷ CFU of B. suis Strain 1330 per gilt. As many gilts as possible were euthanatized and necropsied within eight days prior to their expected date of parturition. Selected tissue samples were collected from all adults and their offspring and cultured on selective media for the isolation and identification of Brucella sp. Eight vaccinated and seven non-vaccinated control animals from the first set of gilts were determined to have been infected with B. suis. None of the gilts in the second set of animals or any of their offspring were determined to have been infected with B. suis. Data from the two sets of animals were not combined. No clinical signs that could be definitively attributed to brucellosis were seen. In contrast to previous studies, there was no evidence in this experiment that oral exposure to B. neotomae protects swine against infection resulting from subsequent conjunctival exposure to B. suis. This may have been a result of failure to provide sufficient exposure of the host to the vaccine organism rather than the inability of the host to produce a protective immune response following exposure to the vaccine organism. | en |
dc.format.medium | electronic | en |
dc.format.mimetype | application/pdf | |
dc.language.iso | en_US | |
dc.publisher | Texas A&M University | |
dc.rights | This thesis was part of a retrospective digitization project authorized by the Texas A&M University Libraries in 2008. Copyright remains vested with the author(s). It is the user's responsibility to secure permission from the copyright holder(s) for re-use of the work beyond the provision of Fair Use. | en |
dc.subject | veterinary pathology. | en |
dc.subject | Major veterinary pathology. | en |
dc.title | Attempted oral vaccination of swine with Brucella neotomae for protection against subsequent challenge with Brucella suis | en |
dc.type | Thesis | en |
thesis.degree.discipline | veterinary pathology | en |
thesis.degree.name | M.S. | en |
thesis.degree.level | Masters | en |
dc.type.genre | thesis | en |
dc.type.material | text | en |
dc.format.digitalOrigin | reformatted digital | en |
Files in this item
This item appears in the following Collection(s)
-
Digitized Theses and Dissertations (1922–2004)
Texas A&M University Theses and Dissertations (1922–2004)
Request Open Access
This item and its contents are restricted. If this is your thesis or dissertation, you can make it open-access. This will allow all visitors to view the contents of the thesis.