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The energy dependence of mutations produced in Escherichia coli by low doses of x-radiation
dc.contributor.advisor | O'Donovan, Gerard A. | |
dc.creator | Bartlett, William Thomas | |
dc.date.accessioned | 2020-08-21T21:47:37Z | |
dc.date.available | 2020-08-21T21:47:37Z | |
dc.date.issued | 1976 | |
dc.identifier.uri | https://hdl.handle.net/1969.1/DISSERTATIONS-472525 | |
dc.description | Vita. | en |
dc.description.abstract | Resistance to the proline analogue, azetidine-2-carboxylic acid, was used as a selection technique with E. coli in order to measure the production of mutations caused by low doses of X-radiation. An improved procedure was required to measure the small changes in the mutation rate. Since the Coulter Counter provided a precise estimate of bacterial numbers, it was found to be essential in this procedure. In addition, a system for producing K X-rays and highly filtered X-rays was developed. This system produced X-rays of good homogeneity whose quality could easily be defined. Bacterial cultures received exposures from 10 R to 138 R at various energies from 29 keV to 88 keV. X-ray exposure at the 29 keV energy produced a significant increase in the bacterial mutation rate. Exposure at 37 keV and 58 keV caused a significant decrease in the bacterial mutation rate. Bacterial cultures showing logarithmic growth were much more sensitive to irradiation than cultures in a non-growing state. Mutants were characterized by reversion with the alkylating agent ethyl methane sulfonate (EMS) and the less specific, but more potent, agent N-methyl-N'-nitro-N-nitrosoguanidine (NTG). EMS reverted the spontaneously occurring mutants and those produced by exposure to 29 keV X-rays more readily than mutants produced by exposure to higher energy X-rays. No correlation was observed with NTG reversion. The data in these experiments indicate that the rate and type of mutations produced by low doses of X-rays were energy dependent. | en |
dc.format.extent | ix, 49 leaves | en |
dc.format.medium | electronic | en |
dc.format.mimetype | application/pdf | |
dc.language.iso | eng | |
dc.rights | This thesis was part of a retrospective digitization project authorized by the Texas A&M University Libraries. Copyright remains vested with the author(s). It is the user's responsibility to secure permission from the copyright holder(s) for re-use of the work beyond the provision of Fair Use. | en |
dc.rights.uri | http://rightsstatements.org/vocab/InC/1.0/ | |
dc.subject | Major biochemistry | en |
dc.subject.classification | 1976 Dissertation B291 | |
dc.subject.lcsh | Escherichia coli | en |
dc.subject.lcsh | Bacterial genetics | en |
dc.subject.lcsh | Experiments | en |
dc.subject.lcsh | X-ray mutagenesis | en |
dc.title | The energy dependence of mutations produced in Escherichia coli by low doses of x-radiation | en |
dc.type | Thesis | en |
thesis.degree.grantor | Texas A&M University | en |
thesis.degree.name | Doctor of Philosophy | en |
dc.contributor.committeeMember | Landmann, Wendell A. | |
dc.contributor.committeeMember | Neff, R. D. | |
dc.contributor.committeeMember | Smith, James D. | |
dc.type.genre | dissertations | en |
dc.type.material | text | en |
dc.format.digitalOrigin | reformatted digital | en |
dc.publisher.digital | Texas A&M University. Libraries | |
dc.identifier.oclc | 2962465 |
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