Identification of trisomes of Sorghum bicolor (L.) Moench by observing progeny of triploid X translocation stocks

Abstract

Triploid (3N) X translocation tester stock (2N) crosses were used to produce F₁ trisomics heterozygous for an interchange for associating the trisomes with the translocated chromosomes (temporarily assigned the letters A to J) in Sorghum bicolor (L.) Moench. The new method was developed and perfected because four of the trisomic F₁ progeny possible from conventional trisomic X translocation tester stock crosses. Over 91% of the progeny from the triploid x translocation tester stock crosses were F₁ plants and were 41.6% were F₁ primary trisomics with an adequate amount of all ten trisomic types. The 10 trisomes of sorghum were determined by cytological observations to be A-bottle brush, B-sparse branch, C-470, D-stiff branch, E-large glume, F-939, G-cone, h-light green, I-small glume and J-10 trisomes.