PCR-aided Amplification: A Tool for the Study of In Vivo Protein-DNA Interactions in the Yeast U6 Promoter

dc.contributor.advisor	Kunkel, Gary R.
dc.creator	Shah, Uma
dc.date.accessioned	2022-04-04T13:49:02Z
dc.date.available	2022-04-04T13:49:02Z
dc.date.issued	1991
dc.identifier.uri	https://hdl.handle.net/1969.1/CAPSTONE-JonesK_1979
dc.description	Program year: 1990/1991	en
dc.description	Digitized from print original stored in HDR	en
dc.description.abstract	The classification of RNA polymerase III transcribed genes into genes with internal promoters (within the coding region) and those with external promoters is no longer a clear one. The S. cerevisiae U6 promoter is comprised of control elements outside the coding region (on the 5' and 3' sides) and a possible internal control element. In the following study I demonstrate the optimization of ligation-mediated PCR for the S. cerevisiae system as a technique that is instrumental in genomic footprinting for determination of protein-DNA interactions in the yeast U6 promoter.	en
dc.format.extent	34 pages	en
dc.format.medium	electronic	en
dc.format.mimetype	application/pdf
dc.subject	S. cerevisiae	en
dc.subject	RNA polymerase III transcribed genes	en
dc.subject	U6 promoter	en
dc.subject	ligation-mediated PCR	en
dc.subject	protein-DNA interactions	en
dc.subject	genomic footprinting	en
dc.title	PCR-aided Amplification: A Tool for the Study of In Vivo Protein-DNA Interactions in the Yeast U6 Promoter	en
dc.type	Thesis	en
thesis.degree.department	Biochemistry and Biophysics	en
thesis.degree.grantor	University Undergraduate Fellow	en
thesis.degree.level	Undergraduate	en
dc.type.material	text	en