PCR-aided Amplification: A Tool for the Study of In Vivo Protein-DNA Interactions in the Yeast U6 Promoter
Abstract
The classification of RNA polymerase III transcribed genes into genes with internal promoters (within the coding region) and those with external promoters is no longer a clear one. The S. cerevisiae U6 promoter is comprised of control elements outside the coding region (on the 5' and 3' sides) and a possible internal control element. In the following study I demonstrate the optimization of ligation-mediated PCR for the S. cerevisiae system as a technique that is instrumental in genomic footprinting for determination of protein-DNA interactions in the yeast U6 promoter.
Description
Program year: 1990/1991Digitized from print original stored in HDR
Subject
S. cerevisiaeRNA polymerase III transcribed genes
U6 promoter
ligation-mediated PCR
protein-DNA interactions
genomic footprinting
Citation
Shah, Uma (1991). PCR-aided Amplification: A Tool for the Study of In Vivo Protein-DNA Interactions in the Yeast U6 Promoter. University Undergraduate Fellow. Available electronically from https : / /hdl .handle .net /1969 .1 /CAPSTONE -JonesK _1979.