Abstract
In order to maximize cell culture growth in bioreactors, biosensors must be developed that are capable of monitoring the cell culture processes non-invasively and continuously. Current techniques used to monitor glucose concentration of cell culture media cannot fulfill these requirements. Therefore a near-infrared spectroscopy system is proposed that can potentially perform the required measurements on-line and without any interaction with the cell culture media. Two types of solutions with varying glucose concentrations were analyzed with the near-infrared spectroscopy system: a glucose and water solution and a bovine serum-based media known as GTSF-2. The results from the glucose and water solution showed the lowest achievable standard deviation of the residuals to be 100.5mg/dl with calibration with one set of data and validation with another set of data from a different day. The serum-based GTSF-2 media, however, seemed to provide an enhancement of the glucose signal despite its more complex formulation. The analysis of the GTSF-2 spectra produced a minimum standard deviation of the residuals of 68.35 mg/dl for analysis of two sets of spectra from the same day. Thus, near-infrared spectroscopy provides a means for glucose concentration measurement.
Galvan, Mark (1994). Near-infrared spectroscopy for the measurement of glucose in an integrated rotating wall vessel. Master's thesis, Texas A&M University. Available electronically from
https : / /hdl .handle .net /1969 .1 /ETD -TAMU -1994 -THESIS -G182.