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dc.contributor.advisorSantschi, Peteren_US
dc.creatorXu, Chenen_US
dc.date.accessioned2010-01-15T00:10:15Zen_US
dc.date.accessioned2010-01-16T00:41:45Z
dc.date.available2010-01-15T00:10:15Zen_US
dc.date.available2010-01-16T00:41:45Z
dc.date.created2007-12en_US
dc.date.issued2009-05-15en_US
dc.identifier.urihttp://hdl.handle.net/1969.1/ETD-TAMU-2566
dc.description.abstractThe extracellular polymeric substances (EPS) of marine bacterium Sagittula stellata and soil bacterium Pseudomonas fluorescens Biovar II, were extracted by six methods referred to the bibliography, efficacies of which were compared based on the EPS yield, composition as well as cell disturbance. Purification methods on these EPS were also improved, which proved to be more cost-effective and involve less interference from broth, compared to previous methods. Size exclusion chromatography (SEC) proved to be a useful tool, providing the “fingerprints” of the EPS extracted by different methods or after each purification step. Studies of the EPS production and composition at different growth stages provided abundant information and a basis for further in-depth studies. Results from SEC demonstrated that bacterial EPS had a constant molecular weight distribution all through the life but with various polymers in different proportions. Three fractions were successfully isolated by a combination of SEC and anion exchange chromatography for “non-attched” EPS produced by Pseudomonas flurorescens Biovar II. Protein turned out to be a major component of EPS in their native states, which was mixed with the broth material and couldn’t be recognized previously. The EPS harvested at the optimal time of the bacterial life was purified according to the improved method and was more enriched in polysaccharides, with small amounts of proteins, giving the molecules amphiphilic properties. In addition, simultaneous determination of neutral sugars and uronic acids by GC-EI-MS provided more information on the monosaccharide composition of the exopolysaccharides. Isoelectric focusing (IEF) spectra of the bacterial EPS spiked with Pu/Th, and Pu-enriched Rocky Flats Environmental Technology Site (RFETS) soil organic colloid spiked with Th showed similar activity distributions of both actinides along the pH gradient, with the activities of both actinides focusing on the low pH region. Characterizations of this Pu-enriched IEF extract from RFETS soil by spectrophotometric methods and ATR-FTIR indicated the co-presence of lipids, proteins and polysaccharides, in contrast to the bacterial EPS, which showed a simpler composition. This suggests that Th/Pu binding to organic macromolecules is more determined by the availability of binding functional groups rather than the exact specific compounds.en_US
dc.format.mediumelectronicen_US
dc.format.mimetypeapplication/pdfen_US
dc.language.isoen_USen_US
dc.subjectextractionen_US
dc.subjectpurificationen_US
dc.subjectcharacterizationen_US
dc.subjectactinide bindingen_US
dc.titleOptimized procedures for extractioin, purification and characterization of exopolymeric substances (eps) from two bacteria (sagittula stellata and pseudomonas fluorescens biovar ii) with relevance to the study of actinide binding in aquatic environmentsen_US
dc.typeBooken
dc.typeThesisen
thesis.degree.departmentOceanographyen_US
thesis.degree.disciplineOceanographyen_US
thesis.degree.grantorTexas A&M Universityen_US
thesis.degree.nameMaster of Scienceen_US
thesis.degree.levelMastersen_US
dc.contributor.committeeMemberAmon, Raineren_US
dc.contributor.committeeMemberCifuentes, Luis Aen_US
dc.type.genreElectronic Thesisen_US
dc.type.materialtexten_US
dc.format.digitalOriginborn digitalen_US


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