dc.contributor.advisor | Yeh, Alvin T. | |
dc.creator | Lee, Anthony Chien-der | |
dc.date.accessioned | 2012-10-19T15:29:26Z | |
dc.date.accessioned | 2012-10-22T17:58:35Z | |
dc.date.available | 2012-10-19T15:29:26Z | |
dc.date.available | 2012-10-22T17:58:35Z | |
dc.date.created | 2011-08 | |
dc.date.issued | 2012-10-19 | |
dc.date.submitted | August 2011 | |
dc.identifier.uri | https://hdl.handle.net/1969.1/ETD-TAMU-2011-08-9871 | |
dc.description.abstract | Nonlinear Optical Microscopy (NLOM) has been shown to be a valuable tool for noninvasive imaging of complex biological systems. An effective approach for multicolor molecular microscopy is simultaneous excitation of multiple fluorophores by broadband sub-10-fs pulses. This dissertation will discuss the development of two spectral imaging systems using the principles of nonlinear optical microscopy for pixel-by-pixel spectral segmentation of multiple fluorescent spectra. The first spectral system is reliant on a fiber-optic cable to transmit fluorescent signal to a spectrometer, while the second is based on a spectrometer with an aberration-corrected concave grating that is directly coupled to the microscope. A photon-counting, 16-channel multianode photomultiplier tube (PMT) is used for both systems.
Custom software developed in LabVIEW controls multiple counter cards as well as a field-programmable gate array (FPGA) for 1 Hz acquisition of 256x256x16 spectral images. Biological specimen consisting of multicolor endothelial cells and zebrafish will be used for experimental verification. Results indicate successful spectral segmentation of multiple fluorophores with a decrease in signal-to-noise ratio in the FPGA-based imaging system. | en |
dc.format.mimetype | application/pdf | |
dc.language.iso | en_US | |
dc.subject | NOMSIS | en |
dc.subject | nonlinear | en |
dc.subject | optical | en |
dc.subject | microscopy | en |
dc.subject | multiphoton | en |
dc.subject | two photon | en |
dc.subject | second harmonic | en |
dc.subject | spectral | en |
dc.subject | imaging | en |
dc.title | Development of the Ultrashort Pulse Nonlinear Optical Microscopy Spectral Imaging System | en |
dc.type | Thesis | en |
thesis.degree.department | Biomedical Engineering | en |
thesis.degree.discipline | Biomedical Engineering | en |
thesis.degree.grantor | Texas A&M University | en |
thesis.degree.name | Doctor of Philosophy | en |
thesis.degree.level | Doctoral | en |
dc.contributor.committeeMember | Holzenburg, Andreas | |
dc.contributor.committeeMember | Maitland, Kristen | |
dc.contributor.committeeMember | Meissner, Kenith | |
dc.type.genre | thesis | en |
dc.type.material | text | en |