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Detection of Rhodococcus equi by polymerase chain reaction using species-specific, non-proprietary primers
| dc.creator | Arriaga, Jose Miguel | |
| dc.date.accessioned | 2012-06-07T23:11:26Z | |
| dc.date.available | 2012-06-07T23:11:26Z | |
| dc.date.created | 2002 | |
| dc.date.issued | 2002 | |
| dc.identifier.uri | https://hdl.handle.net/1969.1/ETD-TAMU-2002-THESIS-A76 | |
| dc.description | Due to the character of the original source materials and the nature of batch digitization, quality control issues may be present in this document. Please report any quality issues you encounter to digital@library.tamu.edu, referencing the URI of the item. | en |
| dc.description | Includes bibliographical references (leaves 22-28). | en |
| dc.description | Issued also on microfiche from Lange Micrographics. | en |
| dc.description.abstract | Species-specific primers for the polymerase chain reaction (PCR) for the detection of Rhodococcus equi were developed. These primers were based on unique DNA fragments produced from R. equi reference strains and field isolates. Following random amplification of polymorphic DNA (RAPD) from R. equi and R. rhodochrous with a set of 40 arbitrary 10-bp primers, a pair of species-specific primers was designed to detect a unique 700-bp fragment of R. equi chromosomal DNA. This PCR product was limited to amplification of DNA from R. equi and was not detectable in DNA extracted from other Rhodococcus species or from a panel of additional gram-positive and gram-negative bacteria. | en |
| dc.format.medium | electronic | en |
| dc.format.mimetype | application/pdf | |
| dc.language.iso | en_US | |
| dc.publisher | Texas A&M University | |
| dc.rights | This thesis was part of a retrospective digitization project authorized by the Texas A&M University Libraries in 2008. Copyright remains vested with the author(s). It is the user's responsibility to secure permission from the copyright holder(s) for re-use of the work beyond the provision of Fair Use. | en |
| dc.subject | veterinary microbiology. | en |
| dc.subject | Major veterinary microbiology. | en |
| dc.title | Detection of Rhodococcus equi by polymerase chain reaction using species-specific, non-proprietary primers | en |
| dc.type | Thesis | en |
| thesis.degree.discipline | veterinary microbiology | en |
| thesis.degree.name | M.S. | en |
| thesis.degree.level | Masters | en |
| dc.type.genre | thesis | en |
| dc.type.material | text | en |
| dc.format.digitalOrigin | reformatted digital | en |
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