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Functional and taxonomic diversity of microbial communities in reclaimed East Texas lignite mine soils
dc.creator | Peach, Allen Edward | |
dc.date.accessioned | 2012-06-07T23:07:39Z | |
dc.date.available | 2012-06-07T23:07:39Z | |
dc.date.created | 2001 | |
dc.date.issued | 2001 | |
dc.identifier.uri | https://hdl.handle.net/1969.1/ETD-TAMU-2001-THESIS-P395 | |
dc.description | Due to the character of the original source materials and the nature of batch digitization, quality control issues may be present in this document. Please report any quality issues you encounter to digital@library.tamu.edu, referencing the URI of the item. | en |
dc.description | Includes bibliographical references (leaves 73-76). | en |
dc.description | Issued also on microfiche from Lange Micrographics. | en |
dc.description.abstract | A two-year study was conducted at Big Brown lignite mine in Freestone County, Texas, to determine the influence of surface mining and reclamation on the functional and taxonomic diversity in soil microbial communities. Quarterly soil samples were collected along a chronosequence including sites of 0, 1, 4, 12, and 28 years following mining and reclamation. In addition to these sites, an unmined reference site, and a tree mott (reclamation age of 20 years) were included in the study. The functional diversity of the microbial communities was assessed using the Biolog sole-carbon source utilization (SCSU) assay. Taxonomic diversity was measured using whole-soil fatty acid methyl ester (FAME) analysis. Results indicated that surface mining had a transient influence on both the functional and taxonomic diversity of the soil microbial communities reducing complexity during disturbance and early reclamation. However, the effect was reversed as the reclamation process matured. Principal component analysis (PCA) was able to separate the younger sites from the older sites in both the SCSU profiles and the FAME profiles of the soils. The separation of sites was greater, however, in the analysis of the FAME profiles suggesting a more significant change in the level of taxonomic diversity. Results from the SCSU analysis revealed a return to similarity with the reference site between one and four years. Fatty acid methyl ester profiles indicated a return to similarity with the reference site in approximately 12 years. | en |
dc.format.medium | electronic | en |
dc.format.mimetype | application/pdf | |
dc.language.iso | en_US | |
dc.publisher | Texas A&M University | |
dc.rights | This thesis was part of a retrospective digitization project authorized by the Texas A&M University Libraries in 2008. Copyright remains vested with the author(s). It is the user's responsibility to secure permission from the copyright holder(s) for re-use of the work beyond the provision of Fair Use. | en |
dc.subject | soil science. | en |
dc.subject | Major soil science. | en |
dc.title | Functional and taxonomic diversity of microbial communities in reclaimed East Texas lignite mine soils | en |
dc.type | Thesis | en |
thesis.degree.discipline | soil science | en |
thesis.degree.name | M.S. | en |
thesis.degree.level | Masters | en |
dc.type.genre | thesis | en |
dc.type.material | text | en |
dc.format.digitalOrigin | reformatted digital | en |
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