| dc.contributor.advisor | Datta, Sumana | |
| dc.creator | Amos, Joseph Edward | |
| dc.date.accessioned | 2013-02-22T20:40:57Z | |
| dc.date.available | 2013-02-22T20:40:57Z | |
| dc.date.issued | 2001 | |
| dc.identifier.uri | https://hdl.handle.net/1969.1/ETD-TAMU-2001-Fellows-Thesis-A49 | |
| dc.description | Due to the character of the original source materials and the nature of batch digitization, quality control issues may be present in this document. Please report any quality issues you encounter to digital@library.tamu.edu, referencing the URI of the item. Digitized from print original stored in HDR. | en |
| dc.description | Includes bibliographical references (leaves 27-28). | en |
| dc.description | Program year: 2000/2001 | en |
| dc.description.abstract | The role of anachronism (ana) protein in stem cell division of Drosophila melanogaster was examined. Synthesis of identifiable ana protein was necessary. The identifying method exploited was that of antibody tagging using a myc epitope or a poly-Histidine region. Analysis of previously utilized complementary DNA (cDNA) sequences showed errors in the alignment of the reading frame. Methods were employed to clone the required ana cDNA sequence into an expression vector (pBAD/Myc-HisA) within the correct reading frame. Restriction endonucleases were then used to confirm that the cDNA sequence and the expression vector had ligated properly. Analysis of the results from the single- and double-restriction digests showed that the ana cDNA (1.6 kilobases) was successfully cloned and ligated into the pBAD/Myc-HisA (4.1 kilobases) expression vector. The ana cDNA was also successfully cloned in frame with the sequence coding for the myc epitope and the poly-Histidine region. This data provides groundwork for the expression of identifiable ana protein. | en |
| dc.format.extent | 36 pages | en |
| dc.format.medium | electronic | en |
| dc.format.mimetype | application/pdf | |
| dc.language.iso | en_US | |
| dc.rights | This thesis was part of a retrospective digitization project authorized by the Texas A&M University Libraries in 2008. Copyright remains vested with the author(s). It is the user's responsibility to secure permission from the copyright holder(s) for re-use of the work beyond the provision of Fair Use. | en |
| dc.subject | stem cell division | en |
| dc.subject | anachronism protein | en |
| dc.subject | complementary DNA | en |
| dc.title | Identifying protein-protein interactions of a cell cycle regulator | en |
| thesis.degree.department | Biochemistry | en |
| thesis.degree.grantor | University Undergraduate Research Fellow | en |
| thesis.degree.name | Fellows Thesis | en |
| thesis.degree.level | Undergraduate | en |
| dc.type.genre | thesis | en |
| dc.type.material | text | en |
| dc.format.digitalOrigin | reformatted digital | en |
