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Differential activation of bovine T lymphocyte subpopulations in response to mitogen and superantigen stimulation
| dc.creator | Keahey, Jennifer Leigh | |
| dc.date.accessioned | 2012-06-07T22:59:52Z | |
| dc.date.available | 2012-06-07T22:59:52Z | |
| dc.date.created | 2000 | |
| dc.date.issued | 2000 | |
| dc.identifier.uri | https://hdl.handle.net/1969.1/ETD-TAMU-2000-THESIS-K42 | |
| dc.description | Due to the character of the original source materials and the nature of batch digitization, quality control issues may be present in this document. Please report any quality issues you encounter to digital@library.tamu.edu, referencing the URI of the item. | en |
| dc.description | Includes bibliographical references (leaves 71-78). | en |
| dc.description | Issued also on microfiche from Lange Micrographics. | en |
| dc.description.abstract | This study examined the response of bovine CD4⁺, CD8⁺, and TCR γ[] T lymphocytes to activation with the mitogens concanavalin A (Con A), phytohemagglutinin (PHA), and pokeweed mitogen (PWM), and the superantigens Staphylococcus enterotoxin A (SEA) and SEB. Several molecules (CD25, CD71, ACT1, PCNA, cyclin D, cdk4, and cdk6) were tested for suitability as activation markers. The marker of activation chosen was CD25, the interleukin-2 receptor α chain. Cells were analyzed by flow cytometry, and the mean fluorescence intensity ratio was determined. A dose response was found to each activating agent in all 3 T cell subsets. A relationship between time in culture with the activating agent and CD25 expression was found to exist in CD4⁺ and TCR []γ cells, but not CD8⁺ cells. This study documents, for the first time, mitogen- and superantigen-induced activation of bovine T cell subsets at early time points. | en |
| dc.format.medium | electronic | en |
| dc.format.mimetype | application/pdf | |
| dc.language.iso | en_US | |
| dc.publisher | Texas A&M University | |
| dc.rights | This thesis was part of a retrospective digitization project authorized by the Texas A&M University Libraries in 2008. Copyright remains vested with the author(s). It is the user's responsibility to secure permission from the copyright holder(s) for re-use of the work beyond the provision of Fair Use. | en |
| dc.subject | veterinary microbiology. | en |
| dc.subject | Major veterinary microbiology. | en |
| dc.title | Differential activation of bovine T lymphocyte subpopulations in response to mitogen and superantigen stimulation | en |
| dc.type | Thesis | en |
| thesis.degree.discipline | veterinary microbiology | en |
| thesis.degree.name | M.S. | en |
| thesis.degree.level | Masters | en |
| dc.type.genre | thesis | en |
| dc.type.material | text | en |
| dc.format.digitalOrigin | reformatted digital | en |
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