Growth and plasmid characteristics of a porcine-derived Salmonella typhimurium resistant to chlortetracycline
Abstract
This study was a preliminary investigation in which porcine derived Salmonella typhimurium was selected for resistance to the equivalent of 200 and 400 grams of chlortetracycline per ton of swine feed. These resistant strains were cultured at 25° C and 37° C, serially diluted, plated, counted, and compared to the non-resistant control. Strain viable cell counts were lower in the resistant strains than in the control. However, viable cell counts in the resistant groups was about the same at both temperatures as were the viable cell counts of the control. Another preliminary study that intended to show the abilities of the resistant strains to transfer resistance to the non-resistant strain gave unexpected results. Plasmids were isolated for the strains (i.e., control, 200 g/ton CT-resistant, and 400 g/ton CT-resistant) and the two resistant that had been cultured in continuous-flow cultures of porcine cecal bacteria. The plasmids were electrophoresed on agarose gels. While all five strains had plasmids, one of the strains cultured in the continuous-flow culture had additional plasmids when not detected in the preparations.
Description
Digitized from print original stored in HDR. Due to the character of the original source materials and the nature of batch digitization, quality control issues may be present in this document. Please report any quality issues you encounter to digital@library.tamu.edu, referencing the URI of the item.Includes bibliographical references: leaves 11-12.
Program year: 1997/1998
Subject
Salmonella typhimuriumchlortetracycline resistance
viable cell counts
resistance transfer
porcine cecal bacteria
plasmids
Citation
Shiflett, J. Mason (1998). Growth and plasmid characteristics of a porcine-derived Salmonella typhimurium resistant to chlortetracycline. University Undergraduate Research Fellow. Available electronically from https : / /hdl .handle .net /1969 .1 /ETD -TAMU -1998 -Fellows -Thesis -S552.