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Serodiagnosis of avian infectious bronchitis virus using recombinant nucleocapsid protein
|dc.description||Due to the character of the original source materials and the nature of batch digitization, quality control issues may be present in this document. Please report any quality issues you encounter to firstname.lastname@example.org, referencing the URI of the item.||en|
|dc.description||Includes bibliographical references: p. 38-48.||en|
|dc.description||Issued also on microfiche from Lange Micrographics.||en|
|dc.description.abstract||A diagnostic immunoaassay using recombinant nucleocapsid protein to detect antibodies to I13V was designed. The nucleocapsid protein was expressed from an expression plasmid, Qiagen pQE8, in E. coli as a fusion protein that was purified on NI-NTA resin. This purified protein was used to determine the optimal conditions for the reagents required in an indirect ELISA and dot blot assay. Based on the optimization of the reagents, a tentative test protocol was derived. 'Me recombinant nucleocapsid protein was found to detect anti-IBV antibodies in polyclonal chicken antisera for various strains of IBV, including Gray, Mass4 1, Conn and Ark99. Negative control serurn with no anti-113V antibodies did not react with the nucleocapsid protein, neither did non-specific proteins like BSA. This study has shown that anti-MV antibodies can be detected with very low concentrations of the recombinant nucleocapsid protein in enzyme immunoassays.||en|
|dc.publisher||Texas A&M University|
|dc.rights||This thesis was part of a retrospective digitization project authorized by the Texas A&M University Libraries in 2008. Copyright remains vested with the author(s). It is the user's responsibility to secure permission from the copyright holder(s) for re-use of the work beyond the provision of Fair Use.||en|
|dc.subject||Major veterinary microbiology.||en|
|dc.title||Serodiagnosis of avian infectious bronchitis virus using recombinant nucleocapsid protein||en|
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