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dc.creatorNdifuna, Abdul-El-Nuru
dc.date.accessioned2012-06-07T22:46:09Z
dc.date.available2012-06-07T22:46:09Z
dc.date.created1996
dc.date.issued1996
dc.identifier.urihttp://hdl.handle.net/1969.1/ETD-TAMU-1996-THESIS-N38
dc.descriptionDue to the character of the original source materials and the nature of batch digitization, quality control issues may be present in this document. Please report any quality issues you encounter to digital@library.tamu.edu, referencing the URI of the item.en
dc.descriptionIncludes bibliographical references: p. 38-48.en
dc.descriptionIssued also on microfiche from Lange Micrographics.en
dc.description.abstractA diagnostic immunoaassay using recombinant nucleocapsid protein to detect antibodies to I13V was designed. The nucleocapsid protein was expressed from an expression plasmid, Qiagen pQE8, in E. coli as a fusion protein that was purified on NI-NTA resin. This purified protein was used to determine the optimal conditions for the reagents required in an indirect ELISA and dot blot assay. Based on the optimization of the reagents, a tentative test protocol was derived. 'Me recombinant nucleocapsid protein was found to detect anti-IBV antibodies in polyclonal chicken antisera for various strains of IBV, including Gray, Mass4 1, Conn and Ark99. Negative control serurn with no anti-113V antibodies did not react with the nucleocapsid protein, neither did non-specific proteins like BSA. This study has shown that anti-MV antibodies can be detected with very low concentrations of the recombinant nucleocapsid protein in enzyme immunoassays.en
dc.format.mediumelectronicen
dc.format.mimetypeapplication/pdf
dc.language.isoen_US
dc.publisherTexas A&M University
dc.rightsThis thesis was part of a retrospective digitization project authorized by the Texas A&M University Libraries in 2008. Copyright remains vested with the author(s). It is the user's responsibility to secure permission from the copyright holder(s) for re-use of the work beyond the provision of Fair Use.en
dc.subjectveterinary microbiology.en
dc.subjectMajor veterinary microbiology.en
dc.titleSerodiagnosis of avian infectious bronchitis virus using recombinant nucleocapsid proteinen
dc.typeThesisen
thesis.degree.disciplineveterinary microbiologyen
thesis.degree.nameM.S.en
thesis.degree.levelMastersen
dc.type.genrethesisen
dc.type.materialtexten
dc.format.digitalOriginreformatted digitalen


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