Abstract
Flourescence in situ hybridization (FISH) was utilized to localize five bovine DNA probes previously mapped in cattle, to chromosomes of four additional diverse bovid species. These species were selected on the basis of proposed chromosomal homologies based on similar banding patterns. Localization of these probes by FISH tested the validity of using interspecific band-based chromosome homologies as assessments of genic homology. All five probes localized to their predicted interspecific homologs, verifying a correlation between banding and genic homology for these loci. Similar FISH techniques were used to hybridize five bovine satellite DNA family probes and one telomeric probe to a distantly related bovid. This expanded our knowledge of bovid comparative chromosomal evolution and organization. Slight differences were noted in hybridization intensity and distribution, which were indicative of interspecific reorganization. The telomere probe showed centromeric hybridization, which supports the involvement of chromosomal fusions in bovid evolution.
Skirpstunas, Peter (1994). Molecular cytogenetic assessments of chromosomal evolution and genome organization within the Bovidae. Master's thesis, Texas A&M University. Available electronically from
https : / /hdl .handle .net /1969 .1 /ETD -TAMU -1994 -THESIS -S628.