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Purification of sulfide oxidase from rat liver
| dc.creator | Pu, Lixia | |
| dc.date.accessioned | 2012-06-07T22:38:04Z | |
| dc.date.available | 2012-06-07T22:38:04Z | |
| dc.date.created | 1994 | |
| dc.date.issued | 1994 | |
| dc.identifier.uri | https://hdl.handle.net/1969.1/ETD-TAMU-1994-THESIS-P976 | |
| dc.description | Due to the character of the original source materials and the nature of batch digitization, quality control issues may be present in this document. Please report any quality issues you encounter to digital@library.tamu.edu, referencing the URI of the item. | en |
| dc.description | Includes bibliographical references. | en |
| dc.description.abstract | The present study represents an initial investigative effort to purify sulfide oxidase from rat liver. Two methods to determine sulfide oxidase activity have been established and both are based on measuring substrate disappearance of sulfide. Both methylene blue and ion selective electrode methods are simple, convenient and accurate. Ultimately the methylene blue method was preferred over the ion selective electrodes method due to its greater sensitivity and smaller size sample requirements. Sulfide oxidase from rat liver has been isolated and purified by means of ammonium sulfate fractionation, anion-exchange chromatography, and size exclusion chromatography. The use of 80% saturation with ammonium sulfate at pH 7.2 provided an initial precipitation of sulfide oxidase, provided an initial precipitation of sulfide oxidase, and after chromatographic procedures a 21 fold purification of the enzyme was obtained. | en |
| dc.format.medium | electronic | en |
| dc.format.mimetype | application/pdf | |
| dc.language.iso | en_US | |
| dc.publisher | Texas A&M University | |
| dc.rights | This thesis was part of a retrospective digitization project authorized by the Texas A&M University Libraries in 2008. Copyright remains vested with the author(s). It is the user's responsibility to secure permission from the copyright holder(s) for re-use of the work beyond the provision of Fair Use. | en |
| dc.subject | medical sciences. | en |
| dc.subject | Major medical sciences. | en |
| dc.title | Purification of sulfide oxidase from rat liver | en |
| dc.type | Thesis | en |
| thesis.degree.discipline | medical sciences | en |
| thesis.degree.name | M.S. | en |
| thesis.degree.level | Masters | en |
| dc.type.genre | thesis | en |
| dc.type.material | text | en |
| dc.format.digitalOrigin | reformatted digital | en |
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