Abstract
The efficacy of administering the following non-antibiotic substances as prophylaxis against Salmonella colonization and/or systemic invasion in mice was evaluated: A) carbohydrates; B) cranberry juice and associated compounds; and C) competitive exclusion cultures. Using these substances, attempts were made to alter the ecological balance of the mouse intestinal microflora so as to interfere with the establishment of Salmonella and thus, increase colonization resistance to an experimental Salmonella challenge in streptomycin-treated and untreated mice. When liquid treatments of the carbohydrates, lactose and lactulose, were administered to the mice, the cecal pH significantly decreased (P < 0.05). The decreased cecal pH caused significantly increased (P < 0.05) concentrations of undissociated volatile fatty acids in the cecal contents. Cecal colonization by Salmonella was not significantly decreased when lactose or lactulose were provided to the mice as compared to the controls. When cranberry juice and an associated compound, hippuric acid, were administered to streptomycin-treated mice, Salmonella cecal colonization was significantly decreased (P < 0.05). Translocation of Salmonella from the intestine to the spleen of the mice was not significantly effected. Use of undefined and defined competitive exclusion cultures derived from the cecal contents of chickens with and without the provision of lactose, and heat-sterilized mice feces combined with the undefined competitive exclusion cultures did not significantly decrease cecal colonization or translocation of Salmonella. None of the treatment substances consistently increased colonization resistance to experimental Salmonella infection in orally challenged mice.
Andrews, Kathleen Ferguson (1993). The in-vivo evaluation of several non-antibotic substances as prophylaxis against Salmonella colonization in mic. Master's thesis, Texas A&M University. Available electronically from
https : / /hdl .handle .net /1969 .1 /ETD -TAMU -1993 -THESIS -A567.