dc.contributor.advisor | Manson, Michael D. | |
dc.creator | Lai, Runzhi | |
dc.date.accessioned | 2010-01-15T00:02:12Z | |
dc.date.accessioned | 2010-01-16T01:38:52Z | |
dc.date.available | 2010-01-15T00:02:12Z | |
dc.date.available | 2010-01-16T01:38:52Z | |
dc.date.created | 2007-05 | |
dc.date.issued | 2009-05-15 | |
dc.identifier.uri | https://hdl.handle.net/1969.1/ETD-TAMU-1337 | |
dc.description.abstract | The key control step in E. coli chemotaxis is regulation of CheA kinase activity by
a set of four transmembrane chemoreceptors. The receptor dimers can form trimeric
complexes (trimers of dimers), and these trimers can be joined by a bridge thought to
consist of a CheW monomer, a CheA dimer, and a second CheW monomer. It has been
proposed that trimers of receptor dimers may be joined by CheW-CheA dimer-CheW
links to form an extended hexagonal lattice that may be the structural basis of the
chemoreceptor patches seen in E. coli. The receptor/CheA/CheW ternary complex is a
membrane-spanning allosteric enzyme whose activity is regulated by protein
interactions. The study presented in this dissertation investigated intermolecular and
intramolecular interactions that affect the chemotactic signal processing. I have
examined functional interactions between the serine receptor Tsr and the aspartate
receptor Tar using a receptor coupled in vitro phosphorylation assay.
The results reveal the emergent properties of mixed receptor populations and
emphasize their importance in the integrated signal processing that underlies bacterial
chemotaxis. A mutational analysis of the extreme C-terminus (last fifty residues) of Tar
is also presented. The results implicate the receptor C-terminus in maintenance of baseline receptor activity and in attractant-induced transmembrane signaling. They also
suggest how adaptive methylation might counteract the effects of attractant binding. | en |
dc.format.medium | electronic | en |
dc.format.mimetype | application/pdf | |
dc.language.iso | en_US | |
dc.subject | Bacterial chemotaxis | en |
dc.subject | Chemoreceptors | en |
dc.subject | Transmembrane signaling | en |
dc.subject | Functional interaction | en |
dc.subject | Receptor C-terminus | en |
dc.title | Signal processing within and between bacterial chemoreceptors | en |
dc.type | Book | en |
dc.type | Thesis | en |
thesis.degree.department | Biology | en |
thesis.degree.discipline | Microbiology | en |
thesis.degree.grantor | Texas A&M University | en |
thesis.degree.name | Doctor of Philosophy | en |
thesis.degree.level | Doctoral | en |
dc.contributor.committeeMember | Benedik, Michael J. | |
dc.contributor.committeeMember | Raushel, Frank M. | |
dc.contributor.committeeMember | Xiong, Jin | |
dc.type.genre | Electronic Dissertation | en |
dc.type.material | text | en |
dc.format.digitalOrigin | born digital | en |