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dc.contributor.advisorCamp, Bennie J.
dc.creatorHill, Dennis Wayne
dc.date.accessioned2020-01-08T17:41:20Z
dc.date.available2020-01-08T17:41:20Z
dc.date.created1976
dc.identifier.urihttps://hdl.handle.net/1969.1/DISSERTATIONS-93384
dc.descriptionIncludes bibliographical references (leaves 91-93)en
dc.description.abstractExtracts of B. multiradiata and H. hoopsii were analyzed by GLC and GC-MS for the presence of a toxic sesquiterpene lactone, hymenoxon. A compound was found in both plants that co-chromatographed with hymenoxon on 3% OV-17 and 3% QF-1 GLC columns and gave a mass spectrum statistically identical to the mass spectrum of hymenoxon. Extracts of both plants were treated with base to convert hymenoxon to psilotropin. Hymenoxon could not be detected in the base treated extracts; however, a compound was detected that co-chromatographed with psilotropin on 3% Ov-17 and 3% QF-1 columns and gave a mass spectrum statistically identical to the mass spectrum of psilotropin. Hymenoxon was successfully extracted with ethyl acetate from ground plant material and quantitatively was analyzed on a 3% Ov-17 GLC column. Dimethoxyhymenoxon or flavone was used as the internal standard. Recovery of fortified hymenoxon from plant tissue was 100-105% and the standard deviation of five analyses was 0.049%. Hymenoxon was shown to react with the sulfhydryl group of cysteine. The second order rate constant for the reaction was 504 liters/mole min. Studies on the reaction of hymenoxon in alkaline solution indicated that at pH 10-12 hymenoxon was converted to intermediates that formed psilotropin and greenein when the pH was made acid. Hymenoxon added to whole sheep blood became sequestered. Evidence was obtained that suggested that hymenoxon was reacting with endogeneous compounds within the erythrocytes..en
dc.format.extentxi, 94 leaves : illustrations, graphsen
dc.format.mediumelectronicen
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.rightsThis thesis was part of a retrospective digitization project authorized by the Texas A&M University Libraries. Copyright remains vested with the author(s). It is the user's responsibility to secure permission from the copyright holder(s) for re-use of the work beyond the provision of Fair Use.en
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/
dc.subjectBiochemistryen
dc.subject.classification1976 Dissertation H646
dc.subject.lcshHymenoxys odoxata DC--Toxicologyen
dc.subject.lcshLivestock poisoning plantsen
dc.subject.lcshVeterinary toxicologyen
dc.subject.lcshPoisonous plantsen
dc.subject.lcshBiochemistryen
dc.titleAnalysis of hymenoxon in plant tissue and the disposition of hymenoxon in the rabbit by Dennis Wayne Hillen
dc.typeThesisen
thesis.degree.grantorTexas A&M Universityen
thesis.degree.nameDoctor of Philosophyen
thesis.degree.levelDoctoralen
thesis.degree.levelDoctorialen
dc.contributor.committeeMemberCreger, C. R.
dc.contributor.committeeMemberCrookshank, H. R.
dc.contributor.committeeMemberDavis, R. H.
dc.type.genredissertationsen
dc.type.materialtexten
dc.format.digitalOriginreformatted digitalen
dc.publisher.digitalTexas A&M University. Libraries


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