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A comparison of the effectiveness of protein denaturing agents for [beta][beta]-lactoglobulin A and Ribonuclease A
| dc.contributor.advisor | Pace, C. N. | |
| dc.creator | Marshall, Henry Frasher | |
| dc.date.accessioned | 2020-08-21T22:17:27Z | |
| dc.date.available | 2020-08-21T22:17:27Z | |
| dc.date.issued | 1975 | |
| dc.identifier.uri | https://hdl.handle.net/1969.1/DISSERTATIONS-776373 | |
| dc.description.abstract | The effectiveness of a variety of protein denaturants has been determined on two proteins, [beta]-lactoglobulin A and Ribonuclease A. These proteins were chosen because they differ substantially in relative hydrophobicity. The proteins were destabilized by the addition of urea, 4.8 M for [beta]-lactoglobulin A, 6.4 M for Ribonuclease A. At these urea concentrations the proteins are just beginning to unfold. Addition of small amounts of a second denaturant produced changes in the levorotation of the proteins; these changes are used to determine a quantitative estimate of the denaturant's potency. With this procedure, denaturation transitions should approach a two-state mechanism and the product formed should approach a randomly-coiled conformation. | en |
| dc.format.extent | 165 leaves | en |
| dc.format.medium | electronic | en |
| dc.format.mimetype | application/pdf | |
| dc.language.iso | eng | |
| dc.rights | This thesis was part of a retrospective digitization project authorized by the Texas A&M University Libraries. Copyright remains vested with the author(s). It is the user's responsibility to secure permission from the copyright holder(s) for re-use of the work beyond the provision of Fair Use. | en |
| dc.rights.uri | http://rightsstatements.org/vocab/InC/1.0/ | |
| dc.subject | Major biochemistry | en |
| dc.title | A comparison of the effectiveness of protein denaturing agents for [beta][beta]-lactoglobulin A and Ribonuclease A | en |
| dc.type | Thesis | en |
| thesis.degree.discipline | Biochemistry | en |
| thesis.degree.grantor | Texas A&M University | en |
| thesis.degree.name | Doctor of Philosophy | en |
| thesis.degree.name | Ph. D. in Biochemistry | en |
| thesis.degree.level | Doctorial | en |
| dc.contributor.committeeMember | Colvin, B. A. | |
| dc.contributor.committeeMember | Dillon, L. S. | |
| dc.contributor.committeeMember | Landmann, W. A. | |
| dc.type.genre | dissertations | en |
| dc.type.material | text | en |
| dc.format.digitalOrigin | reformatted digital | en |
| dc.publisher.digital | Texas A&M University. Libraries | |
| dc.identifier.oclc | 5772569 |
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