Abstract
Three groups of fertilized channel catfish eggs were successfully hatched under controlled laboratory conditions and in a milieu free from pathogenic microorganisms. The effects of plaque purified CCV on fertilized channel catfish eggs and on the fry obtained from these eggs were determined. Fertilized eggs immersed for one hour in a solution containing CCV were refractory to the virus. Fry from CCV-exposed and nonexposed channel catfish eggs were susceptible to CCV exposure. Virus was recovered from fry, both apparently healthy and dead, subjected to the CCV challenge inoculum. The mortality rates observed in this study are not in agreement with published data. The chemical, physical, and biological properties of plaque purified CCV used in this study are in agreement with the criteria established for classifying a virus as a member of the family Herpetoviridae. Virus infectivity was destroyed when treated with lipid solvents. Multiplication of CCV was inhibited by the halogenated nucleosides 5-bromodeoxyuridine and 5-ido-2'-deoxyuridine. Thymidine added to the halogenated nucleoside treated BB cells reversed the action of the inhibitors, allowing CCV to replicate. These data show that the central core of the virus is DNA. In filtration studies the diameter of CCV was estimated to 152 nm. In addition, electron microscope examination of negatively stained enveloped-particles revealed a virus with a diameter of 154 nm, and 95 nm for the nucleocapsid..
Austen, Jack DeWitt (1977). Pathogenesis of channel catfish virus disease and characterization of the virus. Texas A&M University. Texas A&M University. Libraries. Available electronically from
https : / /hdl .handle .net /1969 .1 /DISSERTATIONS -620692.