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Reaction mechanism of the buffer-catalyzed and substituted [beta]-cyclodextrin-catalyzed hydrolysis of catechol cyclic phosphates
dc.contributor.advisor | Hogg, John L. | |
dc.creator | Bokser, Allan David | |
dc.date.accessioned | 2020-09-02T21:01:07Z | |
dc.date.available | 2020-09-02T21:01:07Z | |
dc.date.issued | 1987 | |
dc.identifier.uri | https://hdl.handle.net/1969.1/DISSERTATIONS-26894 | |
dc.description | Typescript (photocopy). | en |
dc.description.abstract | The mechanism for the imidazole-catalyzed hydrolysis of catechol cyclic phosphate has been investigated. The Bronsted β-value obtained for the buffer-catalyzed hydrolyses at a 1:1 buffer ratio was 0.41. The Hammett φ-value obtained for the 1:1-[imidazole]: [imidazolium ion] buffer-catalyzed hydrolysis of substituted catechol cyclic phosphate was 1.08. The proton inventory plots for the imidazole-catalyzed hydrolysis of catechol cyclic phosphate at buffer ratios 75:25, 50:50, and 25:75 [imidazole]: [imidazolium] were dome-shaped, linear, and bowl-shaped, respectively. These different proton inventories indicate that the rate determining step of the reaction changes with the [imidazole]: [imidazolium] ratio and, thus, supports a stepwise mechanism for this reaction. An inverse solvent isotope effect for the hydroxide catalyzed hydrolysis of catechol cyclic phosphate (k(D₂O)/k(H₂O) = 1.63) and the decrease in the normal solvent isotope effects for the imidazole-catalyzed hydrolysis of 4-chlorocatechol cyclic phosphate and 4-nitrocatechol cyclic phosphate (k(H₂O)/k(D₂O) = 1.44, 1.83, respectively) further support this stepwise mechanism. Based on the ³¹P-NMR spectra for the β-cyclodextrin and substituted β-cyclodextrin-catalyzed hydrolysis of 4-tert butyl catechol cyclic phosphate, 6, the reaction occurs with inclusion of the 4-tert butyl catechol cyclic phosphate into the cavity of the cyclodextrin followed by the formation of the product. Heptakis-(2,6-dimethoxy)-β-cyclodextrin and heptakis-(2,3,6-trimethoxy)-β-cyclodextrin were poorer catalysts for the hydrolysis of 6 and 6-mono-imidazole β-cyclodextrin was a better catalyst for the hydrolysis of 6 than β-cyclodextrin. The solvent isotope effects, k(H₂O)/k(D₂O), for the β-cyclodextrin-catalyzed and the 6-mono-imidazole β-cyclodextrin-catalyzed hydrolyses of 6 were 1.35 and 2.35, respectively. Based on the proton inventory for the 6-mono-imidazole β-cyclodextrin-catalyzed hydrolysis of 6, the reaction mechanism is similar to that for the simple model study. | en |
dc.format.extent | xviii, 218 leaves | en |
dc.format.medium | electronic | en |
dc.format.mimetype | application/pdf | |
dc.language.iso | eng | |
dc.rights | This thesis was part of a retrospective digitization project authorized by the Texas A&M University Libraries. Copyright remains vested with the author(s). It is the user's responsibility to secure permission from the copyright holder(s) for re-use of the work beyond the provision of Fair Use. | en |
dc.rights.uri | http://rightsstatements.org/vocab/InC/1.0/ | |
dc.subject | Major chemistry | en |
dc.subject.classification | 1987 Dissertation B686 | |
dc.subject.lcsh | Enzymes | en |
dc.subject.lcsh | Cyclodextrins | en |
dc.subject.lcsh | Catechol | en |
dc.subject.lcsh | Bioorganic chemistry | en |
dc.title | Reaction mechanism of the buffer-catalyzed and substituted [beta]-cyclodextrin-catalyzed hydrolysis of catechol cyclic phosphates | en |
dc.type | Thesis | en |
thesis.degree.discipline | Chemistry | en |
thesis.degree.grantor | Texas A&M University | en |
thesis.degree.name | Doctor of Philosophy | en |
thesis.degree.name | Ph. D. in Chemistry | en |
thesis.degree.level | Doctorial | en |
dc.contributor.committeeMember | Raushel, Frank M. | |
dc.contributor.committeeMember | Sander, Eugene G. | |
dc.contributor.committeeMember | Wong, Chi-Huey | |
dc.type.genre | dissertations | en |
dc.type.material | text | en |
dc.format.digitalOrigin | reformatted digital | en |
dc.publisher.digital | Texas A&M University. Libraries | |
dc.identifier.oclc | 18207307 |
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