Show simple item record

dc.contributor.advisorCook, Benjamin J.
dc.contributor.advisorMagill, Jane M.
dc.creatorWright, Mark Steven
dc.descriptionTypescript (photocopy).en
dc.description.abstractProctolin, a pentapeptide isolated from hindgut tissue of the cockroach L. maderae, stimulates muscular contraction in that organ. It was the intention of this study to show that a second messenger system exists in the hindgut and that proctolin can effect this system. Adenylate cyclase derived from a 10,000 x g homogenate of the hindgut of L. maderae, is stimulated by low Ca²⁺concentrations (less than 100uM), calmodulin, and forskolin. The enzyme is inhibited by elevated Ca²⁺ concentrations (greater than 100 uM) and trifluoperazine. Proctolin is shown not only to bind reversibly to hindgut plasma membranes from L. maderae, but the peptide also has a direct and multiphasic action on adenylate cyclase. The myotropic activity of this peptide regulates hindgut muscle contraction through a second messenger system which involves both adenylate cyclase and calmodulin. The calcium binding protein calmodulin was isolated and characterized from hindgut tissue of L. maderae. The amino acid analysis, molecular weight, and U.V. absorption spectra are compared to calmodulin from bovine brain and Bombyx mori. A protocol for the isolation and purification of calmodulin using reverse phase high performance liquid chromatography was developed.en
dc.format.extentxi, 82 leavesen
dc.rightsThis thesis was part of a retrospective digitization project authorized by the Texas A&M University Libraries. Copyright remains vested with the author(s). It is the user's responsibility to secure permission from the copyright holder(s) for re-use of the work beyond the provision of Fair Use.en
dc.subjectMajor biochemistryen
dc.subject.classification1986 Dissertation W952
dc.subject.lcshPeptide hormonesen
dc.subject.lcshPhysiological effecten
dc.subject.lcshMyoneural junctionen
dc.subject.lcshCalcium-binding proteinsen
dc.titleProctolin, an activator of second messenger systems in insect visceral muscle tissueen
dc.typeThesisen A&M Universityen of Philosophyen D. in Biochemistryen
dc.contributor.committeeMemberGunn, J. M.
dc.contributor.committeeMemberHolman, G. M.
dc.format.digitalOriginreformatted digitalen
dc.publisher.digitalTexas A&M University. Libraries

Files in this item


This item appears in the following Collection(s)

Show simple item record

This item and its contents are restricted. If this is your thesis or dissertation, you can make it open-access. This will allow all visitors to view the contents of the thesis.

Request Open Access