Abstract
Cattle with histologically confirmed ocular squamous cell carcinoma (OSCC) were injected with autochthonous tumor breis in Freund's complete adjuvant. Results from assays used to measure cell-mediated immune responses indicated varying degrees of reactivity. Following injection of the tumor breis an inhibition of migration was observed in two out of four cows with OSCC by the leukocyte migration inhibition assays. Lymphoproliferative responses of isolated peripheral blood lymphocytes using phytomitogens indicated that the cows afflicted with OSCC were immunocompetent. However, no significant lymphoproliferative response was noted when autochthonous tumor breis were used as a stimulant. Lymphocyte medicated cytotoxicity tests, using presumptive neoplastic cells as the antigenic substrate, were not indicative of a cellular immune response. Presumptive neoplastic cells were established in culture using spillout techniques are serum free media. A humoral response to OSCC antigens was not demonstrable using gel precipitation assays, direct fluorescent antibody assays, or indirect fluorescent antibody assays. RNA extracted from lymphoid tissue of cows injected with autochthonous tumor breis did not mediate a transfer or OSCC immunity, but did convert nonsensitive allogeneic peripheral blood lymphocytes to react with tuberculin antigen. RNA extracted from sheep injected with a tumor brei prepared from OSCC tissue did not mediate a xenogenic transfer of tumor immunity or tuberculin sensitivity to bovine peripheral blood lymphocytes.
Lindsay, Gaylord Clark (1976). Immunological investigations with bovine ocular squamous cell carcinoma. Doctoral dissertation, Texas A&M University. Texas A&M University. Libraries. Available electronically from
https : / /hdl .handle .net /1969 .1 /DISSERTATIONS -183396.