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A study of disease symptom development in cultured cells of Nicotiana tabacum L. var. Samsun NN resulting from the direct cellular injection of tobacco mosaic virus
dc.contributor.advisor | Halliwell, Robert S. | |
dc.contributor.advisor | Rosberg, David W. | |
dc.creator | Nims, Robert Cleon | |
dc.date.accessioned | 2020-01-08T18:14:51Z | |
dc.date.available | 2020-01-08T18:14:51Z | |
dc.date.created | 1967 | |
dc.date.issued | 1966 | |
dc.identifier.uri | https://hdl.handle.net/1969.1/DISSERTATIONS-180313 | |
dc.description.abstract | Callus tissue of Nicotiana tabacum var. Samsun NN, a local lesion host of tobacco mosaic virus (TMV), was grown in liquid shake cultures. Three cell chains consisting of 2 to 4 cells each were transferred from the shake cultures to each of 3 different oil chamber microscope slides. In each slide culture a single cell of one chain was directly injected with an aqueous suspension of common TMV that had been passed through a Millipore bacterial filter. A single cell of another chain was injected with a portion of the same inoculum, which had been made noninfective by heating. A third cell chain served as an uninoculated control. Injections were made with a hollow glass needle connected by plastic tubing to a microinjection syringe. The injections were performed with the aid of a micromanipulator. Within 15 minutes after each injection a deposit of material formed at the periphery of the puncture surrounding the base of the needle tip. During the next 36 to 48 hours a thick layer of material was deposited over the entire needle tip, reestablishing the integrity of the cell wall. This permitted removal of the needle without loss of cell contents. ... | en |
dc.format.extent | 69 leaves : illustrations (some color) | en |
dc.format.medium | electronic | en |
dc.format.mimetype | application/pdf | |
dc.language.iso | eng | |
dc.rights | This thesis was part of a retrospective digitization project authorized by the Texas A&M University Libraries. Copyright remains vested with the author(s). It is the user's responsibility to secure permission from the copyright holder(s) for re-use of the work beyond the provision of Fair Use. | en |
dc.rights.uri | http://rightsstatements.org/vocab/InC/1.0/ | |
dc.subject | Plant Pathology | en |
dc.title | A study of disease symptom development in cultured cells of Nicotiana tabacum L. var. Samsun NN resulting from the direct cellular injection of tobacco mosaic virus | en |
dc.type | Thesis | en |
thesis.degree.discipline | Plant Pathology | en |
thesis.degree.grantor | Texas A&M University | en |
thesis.degree.name | Doctor of Philosophy | en |
thesis.degree.level | Doctoral | en |
dc.contributor.committeeMember | Dodd, Jimmie D. | |
dc.contributor.committeeMember | Langston, Ruble G. | |
dc.contributor.committeeMember | McLain, Donald D. | |
dc.type.genre | dissertations | en |
dc.type.material | text | en |
dc.format.digitalOrigin | reformatted digital | en |
dc.publisher.digital | Texas A&M University. Libraries |
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