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The differentiation of clostridial species by gas chromatography and deep agar colony morphology
dc.contributor.advisor | Grumbles, L. C. | |
dc.creator | Ellender, Rudolph Dennis | |
dc.date.accessioned | 2020-09-02T20:45:32Z | |
dc.date.available | 2020-09-02T20:45:32Z | |
dc.date.issued | 1969 | |
dc.identifier.uri | https://hdl.handle.net/1969.1/DISSERTATIONS-174002 | |
dc.description.abstract | An investigation was initiated to determine the feasibility of using gas chromagography and deep agar colony morphology to aid in the identification of clostridial species. Five organisms were studied by gas chromatographic analysis (Clostridium chauvoei, Clostridium hemolyticum, Clostridium novyi type B, Clostridium perfringens type C and Clostridium septicum). The organisms were grown in trypticase soy broth for 24 hours in a special anaerobic system. Cells and spent medium were collected by centrifugation then subjected to fatty acid extraction procedures (chloroform-methanol extraction and saponification). Comparison of the two methods of fatty acid extraction revealed that the saponification method was best for differentiation of the five species tested. Gas chromatographic analysis of the saponified samples yielded fifteen cellular fatty acids. Analysis of variance at the 1% level of significance indicated that eight of the fifteen fatty acids had no significant mean values. A fatty acid profile of each organism was constructed form the statistical results which allowed for differentiation of the five organisms at the species level. Differentiation of clostridial species by comparison of mature deep agar colony forms was demonstrated. ... | en |
dc.format.extent | 122 leaves | en |
dc.format.medium | electronic | en |
dc.format.mimetype | application/pdf | |
dc.language.iso | eng | |
dc.rights | This thesis was part of a retrospective digitization project authorized by the Texas A&M University Libraries. Copyright remains vested with the author(s). It is the user's responsibility to secure permission from the copyright holder(s) for re-use of the work beyond the provision of Fair Use. | en |
dc.rights.uri | http://rightsstatements.org/vocab/InC/1.0/ | |
dc.subject | Major veterinary microbiology | en |
dc.subject.classification | 1969 Dissertation E45 | |
dc.title | The differentiation of clostridial species by gas chromatography and deep agar colony morphology | en |
dc.type | Thesis | en |
thesis.degree.discipline | Veterinary Microbiology | en |
thesis.degree.grantor | Texas A&M University | en |
thesis.degree.name | Doctor of Philosophy | en |
thesis.degree.name | Ph. D. in Veterinary Microbiology | en |
thesis.degree.level | Doctorial | en |
dc.contributor.committeeMember | Foster, B. G. | |
dc.contributor.committeeMember | Hall, C. F. | |
dc.contributor.committeeMember | Hidalgo, R. J. | |
dc.contributor.committeeMember | Prescott, J. M. | |
dc.type.genre | dissertations | en |
dc.type.material | text | en |
dc.format.digitalOrigin | reformatted digital | en |
dc.publisher.digital | Texas A&M University. Libraries | |
dc.identifier.oclc | 5713077 |
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