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The effect of TCDD and related compounds on 17B-estradiol-induced secretion of procathepsin D (52-kDa) protein in MCF-7 human breast cancer cells : mechanistic studies
dc.contributor.advisor | Safe, Stephen H. | |
dc.contributor.advisor | Peterson, David O. | |
dc.creator | Krishnan, Venkatesh | |
dc.date.accessioned | 2024-02-09T20:43:43Z | |
dc.date.available | 2024-02-09T20:43:43Z | |
dc.date.issued | 1994 | |
dc.identifier.uri | https://hdl.handle.net/1969.1/DISSERTATIONS-1554810 | |
dc.description | Vita | en |
dc.description | Major subject: Biochemistry | en |
dc.description | In title, numerals and symbols are used | en |
dc.description.abstract | TCDD is a broad spectrum antiestrogen and mediates its antiestrogenic effects through the aryl hydrocarbon receptor (AhR). 176-estradiol (E2) induces the secretion of procathepsin D, a 52-kDa protein in MCF-7 human breast cancer cells. TCDD inhibits the E2-induced secretion of 52-kDa protein in MCF-7 human breast cancer cells. Initial studies focused on the development of a unique gel staining bioassay for measurement of secreted levels of 52-kDa from MCF-7 human breast cancer cells. This assay was extensively used to investigate the antiestrogenic activity of diverse polychlorinated and polycyclic aromatic hydrocarbons. The results of quantitative structure-activity studies further supported a role for the AhR in mediating inhibition of E2-induced 52-kDa secretion in MCF-7 cells. Furthermore, it was demonstrated that TCDD inhibited E2- induced formation of cytosolic cathepsin-D, cath-D gene transcription and cath-D promoter activity. Subsequent studies demonstrated that E2 regulates cath-D gene transcription through an ER/Spl complex and inhibition of E2-induced gene transcription by TCDD is due to disruption of the ER/Spl complex through a xenobiotic response element which is strategically located between the ER and Spl DNA binding sites. These data suggest a unique mechanism of action for the AhR which acts as a ligand-induced transcription attenuator. This novel mechanism may be involved in TCDD-mediated inhibition of other E2-induced genes. | en |
dc.format.extent | xii, 154 leaves | en |
dc.format.medium | electronic | en |
dc.format.mimetype | application/pdf | |
dc.language.iso | eng | |
dc.rights | This thesis was part of a retrospective digitization project authorized by the Texas A&M University Libraries. Copyright remains vested with the author(s). It is the user's responsibility to secure permission from the copyright holder(s) for re-use of the work beyond the provision of Fair Use. | en |
dc.rights.uri | http://rightsstatements.org/vocab/InC/1.0/ | |
dc.subject | Major biochemistry | en |
dc.title | The effect of TCDD and related compounds on 17B-estradiol-induced secretion of procathepsin D (52-kDa) protein in MCF-7 human breast cancer cells : mechanistic studies | en |
dc.title.alternative | Effect of TCDD and related compounds on seventeen beta-estradiol-induced secretion of procathespin D (fifty two-kDa) protein in MCF-seven human breast cancer cells | en |
dc.type | Thesis | en |
thesis.degree.discipline | Biochemistry | en |
thesis.degree.grantor | Texas A&M University | en |
thesis.degree.name | Doctor of Philosophy | en |
thesis.degree.name | Ph. D | en |
thesis.degree.level | Doctorial | en |
dc.contributor.committeeMember | Kunkel, Gary R. | |
dc.contributor.committeeMember | Giedroc, David P. | |
dc.contributor.committeeMember | Phillips, Timothy D. | |
dc.type.genre | dissertations | en |
dc.type.material | text | en |
dc.format.digitalOrigin | reformatted digital | en |
dc.publisher.digital | Texas A&M University. Libraries | |
dc.identifier.oclc | 34873017 |
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