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Use of human anonymous DNA-segments in bovine gene mapping
| dc.contributor.advisor | Womack, James E. | |
| dc.creator | Weiss, Jutta-Alexandra | |
| dc.date.accessioned | 2024-02-09T20:43:47Z | |
| dc.date.available | 2024-02-09T20:43:47Z | |
| dc.date.issued | 1993 | |
| dc.identifier.uri | https://hdl.handle.net/1969.1/DISSERTATIONS-1531360 | |
| dc.description | Vita | en |
| dc.description | Major subject: Medical Sciences | en |
| dc.description.abstract | The use of human anonymous DNA-segments (D-segments) as probes revolutionized mapping of the human genome. This study investigated the usefulness of twenty-two D-segments as heterologous probes in bovine somatic cell mapping as well as genetic mapping. Six D-segments, D5S6, D5S20, D5S39, D10S20, D11S16, and D12S2 hybridized to unique bovine DNA sequences. D5S6, D5S20, and D5S39 were 100% concordant with one another. D10S20 mapped to U26 (BTA26), D11S16 mapped to U19 (BTA15), and D12S2 was assigned to U3 (BTA5). Four of the six D-segments that hybridized to unique bovine DNA sequences showed RFLPs in cattle. Polymorphism information content (PIC) values calculated for RFLPs found in bovine were generally smaller than those published for human. D5S39 showed the highest PIC value (0.25 with Msp I) and was chosen to be used as a genetic marker in a genetic linkage study in cattle. Three HSA5 gene probes, HEXB, HTR1A, and MAP1B were investigated for their potential to be used as genetic markers with D5S39 in a genetic linkage study in cattle. Somatic cell hybrid analyses found these loci to be 100% concordant with D5S39. The three gene probes showed PIC values between 0.16 and 0.37 in bovine and were therefore deemed suitable as genetic markers in cattle. Linkage analysis of D5S39, HEXB, HTR1A, and MAP1B showed all four genes to be closely linked in bovine. The most likely gene order based on the sum of adjacent lods (SAL) ranking method was calculated to be HEXB-D5S39-MAP1B-HTR1A. Linkage between D5S39 and HEXB as well as D5S39 and MAP1B was found to be conserved between human and bovine. Linkage data entered into the International Bovine Linkage Data Base at the University of Queensland, Australia showed D5S39, HEXB, HTR1A, and MAP1B to be linked to microsatellites on BTA15. The usefulness of human anonymous DNA-segments in bovine gene mapping appears to be limited. Only 27% of the D-segments selected hybridized to unique bovine DNA sequences. Mapped D-segments showed less RFLPs with lower PIC values in bovine compared to those reported in human and, therefore, appear to be less useful as markers for genetic linkage analysis in bovine. | en |
| dc.format.extent | xii, 86 leaves | en |
| dc.format.medium | electronic | en |
| dc.format.mimetype | application/pdf | |
| dc.language.iso | eng | |
| dc.rights | This thesis was part of a retrospective digitization project authorized by the Texas A&M University Libraries. Copyright remains vested with the author(s). It is the user's responsibility to secure permission from the copyright holder(s) for re-use of the work beyond the provision of Fair Use. | en |
| dc.rights.uri | http://rightsstatements.org/vocab/InC/1.0/ | |
| dc.subject | Major medical sciences | en |
| dc.subject.classification | 1993 Dissertation W431 | |
| dc.title | Use of human anonymous DNA-segments in bovine gene mapping | en |
| dc.type | Thesis | en |
| thesis.degree.discipline | Medical Sciences | en |
| thesis.degree.grantor | Texas A&M University | en |
| thesis.degree.name | Doctor of Philosophy | en |
| thesis.degree.name | Ph. D | en |
| thesis.degree.level | Doctorial | en |
| dc.contributor.committeeMember | Elder, Robert T. | |
| dc.contributor.committeeMember | Ficht, Allison R. | |
| dc.contributor.committeeMember | Templeton, Joe W. | |
| dc.type.genre | dissertations | en |
| dc.type.material | text | en |
| dc.format.digitalOrigin | reformatted digital | en |
| dc.publisher.digital | Texas A&M University. Libraries | |
| dc.identifier.oclc | 34555917 |
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