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dc.contributor.advisorWomack, James
dc.creatorTobin, Tammy Christine
dc.date.accessioned2024-02-09T20:43:46Z
dc.date.available2024-02-09T20:43:46Z
dc.date.issued1990
dc.identifier.urihttps://hdl.handle.net/1969.1/DISSERTATIONS-1197477
dc.descriptionTypescript (photocopy)en
dc.descriptionVitaen
dc.description"Major subject: Geneticsen
dc.description.abstractBovine genomic IGL and IGHM probes were used to map these immunoglobulin genes to bovine syntenic group U23 and BTA21 using bovine-hamster hybrid somatic cells. These cells segregate bovine chromosomes while retaining a full complement of the hamster genome. A human IGHG4 probe was used to map the immunoglobulin heavy chain gamma constant region to bovine chromosome 21 (syntenic group U4). FOS, FES, PDGFB, IL1, SCN2A, and MYCN were also mapped to bovine syntenic groups, and uncovered many interesting areas of evolutionary differences between bovine, human and murine syntenic groups. The syntenic assignments also allowed predictions to be made regarding the chromosomal location of the gene markers on human and mouse chromosomes. The probes used for mapping were used to screen cattle of several different breeds for restriction fragment length polymorphisms (RFLPs). It is hoped that the polymorphisms generated by this study will be linked to traits of economic importance, and, as such, can be used in breeding programs. The IGHG4 probe was used to screen a bovine sperm DNA library for homologous sequences. Three clones were isolated, two of which appeared to be identical based on restriction analysis. Restriction maps are reported for the clones. Further analysis of the clones through sequencing, in addition to mapping more markers to the bovine syntenic groups containing the immunoglobulin genes, will add to our knowledge of mammalian genome evolution, and the evolution of the immunoglobulin genes in particular.en
dc.format.extentxiii, 122 leavesen
dc.format.mediumelectronicen
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.rightsThis thesis was part of a retrospective digitization project authorized by the Texas A&M University Libraries. Copyright remains vested with the author(s). It is the user's responsibility to secure permission from the copyright holder(s) for re-use of the work beyond the provision of Fair Use.en
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/
dc.subjectMajor geneticsen
dc.subject.classification1990 Dissertation T629
dc.subject.lcshCattleen
dc.subject.lcshImmunologyen
dc.subject.lcshGenetic aspectsen
dc.subject.lcshGene mappingen
dc.subject.lcshImmunoglobulin genesen
dc.titleComparative mapping of the immunoglobulin genes in domestic cattleen
dc.typeThesisen
thesis.degree.disciplineGeneticsen
thesis.degree.grantorTexas A&M Universityen
thesis.degree.nameDoctor of Philosophyen
thesis.degree.namePh. Den
thesis.degree.levelDoctorialen
dc.contributor.committeeMemberPeterson, David O.
dc.contributor.committeeMemberSkow, Loren C.
dc.contributor.committeeMemberTempleton, Joe W.
dc.type.genredissertationsen
dc.type.materialtexten
dc.format.digitalOriginreformatted digitalen
dc.publisher.digitalTexas A&M University. Libraries
dc.identifier.oclc24853201


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