Activity Of Transfer RNA Methyltransferases During Insect Metamorphosis
Abstract
Transfer RNA (tRNA) methyltransferases were partially purified from Manduca sexta prepupa larvae and 2-day-old pupae. The purification procedure involved pH 5 precipitation, gel filtration to remove endogenous inhibitors and activators, and ammonium sulfate precipitation. To ensure maximal activation of all preparations, 0.36 M ammonium acetate was used in the reaction mixture. Escherichia coli B tRNA was the methyl accepting substrate, and ¹⁴CH₃-S-adenosylmethionine was the methyl donor. The prepupa larvae had a specific activity of 140 units/mg protein. The 2-day-old pupae had a specific activity of 430 units/mg protein.
Description
Program year: 1976/1977Digitized from print original stored in HDR
Citation
Alexander, Victor Charles (1977). Activity Of Transfer RNA Methyltransferases During Insect Metamorphosis. University Undergraduate Fellow. Available electronically from https : / /hdl .handle .net /1969 .1 /CAPSTONE -SykoraE _1998.