In Vitro Propagation of Thorny Blackberries
Abstract
Tissue culture of 3 newly released thorny blackberry cultivars, ‘Womack’, 'Rosborough', and 'Brison' was studied for sterilization procedure, shoot and root development, and plantlet acclimatization. Best sterilization procedure was found to be a 10-15 sec dip in 30% ethanol, then rinsed in double distilled water. Under the laminar flow hood, the explants were placed in a 5 min dip in 0.25% sodium hypochlorite plus Tween-20 and rinsed 3 times in double distilled sterile water. For shoot development media, 4 types of explants were cultured in a basic Murashige and Skoog salt mixture plus 0.4 mg/l myo-inositol, 0.1 mg/l GA3, 30 g/l sucrose, 8 g/l agar, 0.1 mg/l BA, and 0.1 mg/l IBA. Stem cuttings had less % contamination and longer shoot length. Shoots developed after 4 days in culture and once reached a size of 1 cm, they were transferred to a rooting media of 0, 1, 3, 6, 10 mg/l IBA. Optimum concentration to induce rooting was 3 mg/l IBA. Immediately after root initiation, plantlets were transferred to a medium with no growth regulators. Plantlets 5 cm in length were transplanted to small plastic pots containing 1 peat: 1 perlite (v/v). Acclimatization procedure followed a weekly exchange of a series of polyethylene covers with 0, 15, 50, and 98 perforations. Following this procedure, 100% of plantlets survived.
Description
Program year: 1980-1981Digitized from print original stored in HDR
Subject
thorny blackberry cultivarssterilization
shoot and root development
plantlet acclimatization
Citation
Ringhoffer, Karen Gail (1981). In Vitro Propagation of Thorny Blackberries. University Undergraduate Fellows. Available electronically from https : / /hdl .handle .net /1969 .1 /CAPSTONE -RinghofferK _1981.