| dc.description.abstract | Gross observation of testicular parenchyma of prepubertal horses reveals both light and
dark regions. The light regions are associated with localized initiation and regulation of
spermatogenesis. It is hypothesized that different proteins and protein expression levels
in the light regions are factors contributing to successful sperm production. Differential
Gel Electrophoresis (DIGE) and DeCyder Image analysis of testicular extracts from
prepubertal stallions has identified forty proteins that may be involved in the regulation
of the onset of spermatogenesis. Preliminary LC/MS/MS mass spectrometric analysis of
six protein spots has been analyzed, but no protein identifications have yet been made.
The results of this preliminary research are inconclusive with respect to protein
identification, but the developments of protocols for procurement, protein extraction of
light and dark tissue and DIGE analysis have been successful. | en |
