In Vitro Culture of 'Dog Ridge' Grapevine
Abstract
Vitis champini ‘Dog Ridge’ grapevine is a potentially desirable rootstock for Texas
grapevines because of its disease resistance. This selection is reported to be difficult to
root through hardwood cuttage as is often practiced for grapevine. A study was
undertaken to establish a protocol to propagate ‘Dog Ridge’ grapevine in vitro by
comparing combinations of explant type, basal salts, and benzyladenine (BA)
concentration to proliferate shoots followed by in vitro and ex vitro rooting. Shoot tip
and axillary bud explants were harvested from actively growing stock plants, disinfested
with 10% v/v Clorox, rinsed in sterile distilled water and cultured on either Murashige
and Skoog (MS) or Woody Plant Medium (WPM) containing 0, 4.4 and 8.8 μM BA for
12 weeks. Axillary bud explants cultured on MS medium proliferated better than shoot
tips. Axillary bud explants cultured on media containing 4.4 or 8.8 μM BA proliferated
better than shoot tip explants regardless of the BA concentration in the medium. Tissue
cultured shoots rooted in either WPM medium without BA in vitro or in Redi-earth® potting mix ex vitro. Shoots developed roots in vitro better than under ex vitro
conditions.
Citation
Wong, Kah-Yat Isaac (2009). In Vitro Culture of 'Dog Ridge' Grapevine. Available electronically from https : / /hdl .handle .net /1969 .1 /86500.