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dc.contributor.advisorPietrantonio, Patricia V.
dc.creatorHolmes, Steven P.
dc.date.accessioned2004-09-30T01:41:13Z
dc.date.available2004-09-30T01:41:13Z
dc.date.created2003-12
dc.date.issued2004-09-30
dc.identifier.urihttps://hdl.handle.net/1969.1/60
dc.description.abstractMyokinins are invertebrate neuropeptides with myotropic and diuretic activity. The lymnokinin receptor from the snail Lymnaea stagnalis was the only previously identified myokinin receptor. A cDNA encoding a neuropeptide receptor was cloned from the southern cattle tick, Boophilus microplus. The deduced amino acid sequence was 40 % identical to the lymnokinin receptor. The receptor transcript is present in all tick life stages as determined by semiquantitative RT-PCR. When expressed in mammalian CHO-K1 cells, myokinins at nanomolar concentrations induced increases in intracellular calcium as measured by fluorescent cytometry. The rank order of potency for peptides tested was FFFSWS-NH2≥FFFSWG-NH2≥FFSWG-NH2>FYSWG-NH2>muscakinin>lymnokinin>>APTGFFGVR-NH2. The receptor coupled to a pertussis toxin insensitive G protein. Absence of extracellular calcium did not inhibit the calcium response, indicating the release of Ca2+ from intracellular stores. Receptor transcript was detected by RT-PCR in the dissected synganglia, ovaries, salivary glands, guts and Malpighian tubules of partially engorged adult female ticks. It is concluded that the B. microplus receptor is the first myokinin receptor cloned from an arthropod, and the first neuropeptide receptor known from the Acari. The presence of this receptor transcript in multiple tissues and all life stages suggests a multifunctional role in ticks.en
dc.format.extent7515982 bytesen
dc.format.extent204011 bytesen
dc.format.mediumelectronicen
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherTexas A&M University
dc.subjectBoophilus microplusen
dc.subjectGPCRen
dc.subjectneuropeptide receptoren
dc.subjectmyokininen
dc.titleThe characterization, functional expression, and localization of the first arthropod myokinin receptor from the southern cattle tick, Boophilus microplus (Acari: ixodidae)en
dc.typeBooken
dc.typeThesisen
thesis.degree.departmentEntomologyen
thesis.degree.disciplineEntomologyen
thesis.degree.grantorTexas A&M Universityen
thesis.degree.nameDoctor of Philosophyen
thesis.degree.levelDoctoralen
dc.contributor.committeeMemberBurghardt, Robert C.
dc.contributor.committeeMemberChen, Andrew C.
dc.contributor.committeeMemberIng, Nancy H.
dc.contributor.committeeMemberTeel, Pete D.
dc.type.genreElectronic Dissertationen
dc.type.materialtexten
dc.format.digitalOriginborn digitalen


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