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dc.contributor.advisorVinson, S. Bradleighen_US
dc.creatorHale, Michael Walkeren_US
dc.date.accessioned2007-09-17T19:37:42Z
dc.date.available2007-09-17T19:37:42Z
dc.date.created2003-05en_US
dc.date.issued2007-09-17
dc.identifier.urihttp://hdl.handle.net/1969.1/5910
dc.description.abstractThelohania solenopsae Knell, Allen and Hazard is a microsporidian pathogen that infects the red imported fire ant Solenopsis invicta Buren. This five part study examined the effects that T. solenopsae has on constructs of colony fitness in field mounds and adoption rates, how T. solenopsae spores are affected by different stable temperatures. This study also examined the effects on T. solenopsae spores due to centrifugation out of the host cell, pH of the solution the spores are kept, and food classes that could be ingested by S. invicta. For the first study, a total of 29 colonies were collected, 16 infected, and 13 uninfected. The study concluded significantly lower brood production in uninfected field colonies when compared to infected field colonies. Additionally, the total number of queens per colony is significantly greater in T. solenopsae infected mounds with 35.4±31.9 queens for infected mounds and 15.5±11.2 queens for uninfected mounds, demonstrating a possible host response to parasite infection. In the study examining stable temperature effects on T. solenopsae spore growth and spore type, a stable temperature of 31°C induced greater production of binucleate free spores in worker S. invicta. There was an overall decrease of T. solenopsae octospores at 16°C, 26°C and 31°C. The studies concerning extraction of T. solenopsae spores, and inclusion in food infusions demonstrated viable spore recovery after centrifugation, verified by using Calcofluor M2R and Sytox Green dual staining. This study demonstrated significant decreases in spore viability over a 10 week period. The study concerning pH demonstrated significant effects of differing pH parameters with the best viable spore recovery at pH 4 and no spore recovery at pH 1 and pH 2. The least viable spore recovery occurred at pH 6 and pH 12 after 24 hours of emersion verified by using Calcofluor M2R and Sytox Green dual staining.en_US
dc.format.extent718985 bytes
dc.format.mediumelectronicen_US
dc.format.mimetypeapplication/pdf
dc.language.isoen_USen_US
dc.publisherTexas A&M Universityen_US
dc.subjectSolenopsis invictaen_US
dc.subjectThelohania solenopsaeen_US
dc.subjectfitnessen_US
dc.titleHost/parasite interactions between Solenopsis invicta (Hymenoptera: Formicidae) and Thelohania solenopsae (Microsporida: Thelohaniidae)en_US
dc.typeBooken
dc.typeThesisen
thesis.degree.departmentEntomologyen_US
thesis.degree.disciplineEntomologyen_US
thesis.degree.grantorTexas A&M Universityen_US
thesis.degree.nameMaster of Scienceen_US
thesis.degree.levelMastersen_US
dc.contributor.committeeMemberBernal, Julioen_US
dc.contributor.committeeMemberPeterson, Markusen_US
dc.type.genreElectronic Thesisen_US
dc.type.materialtexten_US
dc.format.digitalOriginborn digitalen_US


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