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dc.contributor.advisorLupton, Joanne R.
dc.creatorCovert, Kristy Lynn
dc.date.accessioned2006-08-16T19:11:08Z
dc.date.available2006-08-16T19:11:08Z
dc.date.created2003-05
dc.date.issued2006-08-16
dc.identifier.urihttp://hdl.handle.net/1969.1/3958
dc.description.abstractWe have previously shown that dietary fish oil and fiber, particularly the highly-fermentable pectin, are protective against colon cancer in a rat model of carcinogenesis. Therefore, based upon the current body of literature and our previous experimental findings, we hypothesized that one mechanism by which dietary fish oil+pectin suppress the promotion stage of colon cancer is through butyrate, the fermentation product of fiber, targeting (in particular) the p21Waf1/Cip1 gene and, via targeted histone hyperacetylation, inducing its expression. We found that dietary butyrate supplementation increased the concentration of fecal butyrate (mole %) in the distal colon, and that this increase corresponded to an increase in histone H4 acetylation. Similarly, diets supplemented with butyrate increased p21Waf1/Cip1 expression despite azoxymethane (AOM) treatment, which was not seen in non-butyrate supplemented diets. Furthermore, fish oil+butyrate diets resulted in the highest levels of apoptosis and the lowest levels of ACF, while corn oil+butyrate diets resulted in the lowest levels of apoptosis and the highest levels of ACF. Thus, it appears that the protective effect of fish oil+butyrate is due to the unique properties of fish oil, providing an environment in which butyrateÂ’s enhancement of histone acetylation and p21 expression are pro-apoptotic, thereby diminishing pre-neoplastic ACF development.en
dc.format.extent608675 bytesen
dc.format.mediumelectronicen
dc.format.mimetypeapplication/pdf
dc.language.isoen_US
dc.publisherTexas A&M University
dc.subjectFish Oilen
dc.subjectButyrateen
dc.subjectColonen
dc.subjectHistoneen
dc.titleDietary fish oil and butyrate increase apoptosis and decrease aberrant crypt foci in colon cancer by enhancing histone acetylation and p21waf1/cip1 expressionen
dc.typeBooken
dc.typeThesisen
thesis.degree.departmentAnimal Scienceen
thesis.degree.disciplineNutritionen
thesis.degree.grantorTexas A&M Universityen
thesis.degree.nameMaster of Scienceen
thesis.degree.levelMastersen
dc.contributor.committeeMemberChapkin, Robert S.
dc.contributor.committeeMemberHood, David M.
dc.contributor.committeeMemberTurner, Nancy D.
dc.type.genreElectronic Thesisen
dc.type.materialtexten
dc.format.digitalOriginborn digitalen


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